Optimization Manufacture of Virus- and Tumor-Specific T Cells

被引：14

作者：

Lapteva, Natalia ^{[1
]}

Vera, Juan F. ^{[1
]}

机构：

[1] Baylor Coll Med, Ctr Cell & Gene Therapy, Houston, TX 77030 USA

来源：

STEM CELLS INTERNATIONAL | 2011年 / 2011卷

关键词：

ANTIGEN-PRESENTING CELLS; HOLLOW-FIBER BIOREACTOR; ADOPTIVE TRANSFER; SUICIDE-GENE; EX-VIVO; LYMPHOCYTES; IMMUNOTHERAPY; INFUSION; LYMPHOMA; THERAPY;

D O I：

10.4061/2011/434392

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Although ex vivo expanded T cells are currently widely used in pre-clinical and clinical trials, the complexity of manufacture remains a major impediment for broader application. In this review we discuss current protocols for the ex vivo expansion of virus- and tumor-specific T cells and describe our experience in manufacture optimization using a gas-permeable static culture flask (G-Rex). This innovative device has revolutionized the manufacture process by allowing us to increase cell yields while decreasing the frequency of cell manipulation and in vitro culture time. It is now being used in good manufacturing practice (GMP) facilities for clinical cell production in our institution as well as many others in the US and worldwide.

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