Experimental and theoretical analysis for improved microscope design of optical projection tomographic microscopy

被引：3

作者：

Coe, Ryan L. ^{[1
]}

Seibel, Eric J. ^{[2
]}

机构：

[1] Univ Washington, Dept Bioengn, Seattle, WA 98105 USA

[2] Univ Washington, Dept Mech Engn, Seattle, WA 98105 USA

来源：

OPTICS LETTERS | 2013年 / 38卷 / 17期

基金：

美国国家科学基金会;

关键词：

TIME-DOMAIN METHOD; 3D;

D O I：

10.1364/OL.38.003398

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present theoretical and experimental results of axial displacement of objects relative to a fixed condenser focal plane (FP) in optical projection tomographic microscopy (OPTM). OPTM produces three-dimensional, reconstructed images of single cells from two-dimensional projections. The cell rotates in a microcapillary to acquire projections from different perspectives where the objective FP is scanned through the cell while the condenser FP remains fixed at the center of the microcapillary. This work uses a combination of experimental and theoretical methods to improve the OPTM instrument design. (C) 2013 Optical Society of America

引用

页码：3398 / 3401

页数：4

共 11 条

[1] Aberration-free optical refocusing in high numerical aperture microscopy [J].