Development of a heterologous gene expression system for use in Lactococcus lactis

被引：0

作者：

Bredmose, L ^{[1
]}

Madsen, SM ^{[1
]}

Vrang, A ^{[1
]}

Ravn, P ^{[1
]}

Johnsen, MG ^{[1
]}

Glenting, J ^{[1
]}

Arnau, J ^{[1
]}

Israelsen, H ^{[1
]}

机构：

[1] Inst Biotechnol, Sector Appl Mol Biol, DK-2970 Horsholm, Denmark

来源：

RECOMBINANT PROTEIN PRODUCTION WITH PROKARYOTIC AND EUKARYOTIC CELLS: A COMPARATIVE VIEW ON HOST PHYSIOLOGY | 2001年

关键词：

Lactococcus lactis; lactic acid bacteria; Gram-positive; heterologous gene expression; recombinant proteins; P170; promoter; secretion; vaccine antigens;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a gene expression system for use in the lactic acid bacterium Lactococcus lactis. The expression is controlled by the L. lactis promoter, P170, which is up-regulated by low-pH during the transition to stationary phase. The P170 promoter is coupled to a modified L. lactis signal peptide, SP310mut2, to mediate secretion of the recombinant protein. Optimisation of genetic components, growth conditions and medium composition has resulted in the production of approximately 300 mg of secreted protein per litre culture. We have successfully produced a number of heterologous proteins from both prokaryotic and eukaryotic organisms. The system is currently used for the production of bacterial vaccine components for use in humans.

引用

页码：269 / 275

页数：7