Characteristics of carbapenem-resistant Enterobacteriaceae isolates from Korea

被引：37
作者：
Kim, So Yeon [1 ]
Shin, Juyoun [1 ]
Shin, Sang Yop [2 ]
Ko, Kwan Soo [1 ]
机构：
[1] Sungkyunkwan Univ, Sch Med, Samsung Biomed Res Inst, Dept Mol Cell Biol, Suwon 440746, South Korea
[2] Korea Med Inst, Seoul 150896, South Korea
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2013年 / 76卷 / 04期
关键词：
NDM-1; ST11; Polymyxins; Tigecycline; Klebsiella pneumoniae; KLEBSIELLA-PNEUMONIAE; DISSEMINATION; INFECTIONS; PREVENTION; PATIENT; CLONES; OMPK36; ST11;
D O I：
10.1016/j.diagmicrobio.2013.04.006
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
In this study, the characteristics of carbapenem-resistant Enterobacteriaceae (CRE) isolates from Korea was investigated. A total of 22 CRE isolates were investigated, and most were identified as Klebsiella pneumoniae (16 isolates). In vitro antimicrobial susceptibility testing, multilocus sequence typing, and pulsed-field gel electrophoresis were performed. Extended-spectrum beta-lactamase (ESBL) and carbapenemase genes were detected using gene amplification and sequencing. Efflux pump activity and inactivating mutations in OmpK35/36 were also investigated. Among 22 CRE isolates, only 5 produced metallo-beta-lactamases (3 NDM-1, one VIM-2 and one IMP-1). Four and 2 K. pneumoniae and Serratia marcescens isolates showed resistance to polymyxins, respectively, and 2 CRE isolates (1 K. pneumoniae and C. freundii) were resistant to tigecycline. The prevalent carbapenem resistance mechanism found in K. pneumoniae might be porin defects. The most prevalent clone of carbapenem-resistant K. pneumoniae was ST11 (56.3%), which is the most frequently identified clone among ESBL-producing K. pneumoniae isolates from Korea. Three NDM-1-producing K. pneumoniae isolates belonged to a single clone (ST340) despite their different antimicrobial susceptibilities. In the present study, the clonal dissemination of carbapenem-resistant K. pneumoniae isolates (ST11) and NDM-1-producing K. pneumoniae isolates (ST340) was determined. Polymyxin- and tigecycline-resistant CRE isolates were also identified, which limits treatment options for infections causes by these organisms. (c) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码：486 / 490
页数：5
相关论文
共 26 条
[11]   Contribution of OmpK36 to carbapenem susceptibility in KPC-producing Klebsiella pneumoniae [J].
Landman, David ;
Bratu, Simona ;
Quale, John .
JOURNAL OF MEDICAL MICROBIOLOGY, 2009, 58 (10) :1303-1308
[12]   Major uropathogenic Escherichia coli strain isolated in the northwest of England identified by multilocus sequence typing [J].
Lau, Siu Ha ;
Reddy, Suganya ;
Cheesbrough, John ;
Bolton, Frederick J. ;
Willshaw, Geraldine ;
Cheasty, Tom ;
Fox, Andrew J. ;
Upton, Mathew .
JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (03) :1076-1080
[13]   EUCAST expert rules in antimicrobial susceptibility testing [J].
Leclercq, R. ;
Canton, R. ;
Brown, D. F. J. ;
Giske, C. G. ;
Heisig, P. ;
MacGowan, A. P. ;
Mouton, J. W. ;
Nordmann, P. ;
Rodloff, A. C. ;
Rossolini, G. M. ;
Soussy, C. -J. ;
Steinbakk, M. ;
Winstanley, T. G. ;
Kahlmeter, G. .
CLINICAL MICROBIOLOGY AND INFECTION, 2013, 19 (02) :141-160
[14]   High prevalence of CTX-M-15-producing Klebsiella pneumoniae isolates in Asian countries: diverse clones and clonal dissemination [J].
Lee, Mi Young ;
Ko, Kwan Soo ;
Kang, Cheol-In ;
Chung, Doo Ryeon ;
Peck, Kyong Ran ;
Song, Jae-Hoon .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2011, 38 (02) :160-163
[15]   Dissemination of ST131 and ST393 community-onset, ciprofloxacin-resistant Escherichia coli clones causing urinary tract infections in Korea [J].
Lee, Mi Young ;
Choi, Hyeon Jin ;
Choi, Ji Young ;
Song, Minsuk ;
Song, Yoosuk ;
Kim, Shin-Woo ;
Chang, Hyun-Ha ;
Jung, Sook-In ;
Kim, Yeon-Sook ;
Ki, Hyun Kyun ;
Son, Jun Seong ;
Kwon, Ki Tae ;
Heo, Sang Taek ;
Yeom, Joon-Sup ;
Shin, Sang Yop ;
Chung, Doo Ryeon ;
Peck, Kyong Ran ;
Song, Jae-Hoon ;
Ko, Kwan Soo .
JOURNAL OF INFECTION, 2010, 60 (02) :146-153
[16]   Colistin in the 21st century [J].
Nation, Roger L. ;
Li, Jian .
CURRENT OPINION IN INFECTIOUS DISEASES, 2009, 22 (06) :535-543
[17]   Carbapenem resistance in Enterobacteriaceae: here is the storm! [J].
Nordmann, Patrice ;
Dortet, Laurent ;
Poirel, Laurent .
TRENDS IN MOLECULAR MEDICINE, 2012, 18 (05) :263-272
[18]   Global Spread of Carbapenemase-producing Enterobacteriaceae [J].
Nordmann, Patrice ;
Naas, Thierry ;
Poirel, Laurent .
EMERGING INFECTIOUS DISEASES, 2011, 17 (10) :1791-1798
[19]   The characteristics of NDM-producing Klebsiella pneumoniae from Canada [J].
Peirano, Gisele ;
Pillai, Dylan R. ;
Pitondo-Silva, Andre ;
Richardson, David ;
Pitout, Johann D. D. .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2011, 71 (02) :106-109
[20]   Tigecycline efflux as a mechanism for nonsusceptibility in Acinetobacter baumannii [J].
Peleg, Anton Y. ;
Adams, Jennifer ;
Paterson, David L. .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2007, 51 (06) :2065-2069
123