Enhanced reconstruction of structured illumination microscopy on a polarized specimen

被引：10

作者：

Chen, Xingye ^{[1
]}

Zhanghao, Karl ^{[2
,3
]}

Li, Meiqi ^{[3
]}

Qiao, Chang ^{[1
]}

Liu, Wenhui ^{[1
]}

Xi, Peng ^{[2
,3
]}

Dai, Qionghai ^{[1
,4
]}

机构：

[1] Tsinghua Univ, Dept Automat, Beijing, Peoples R China

[2] Southern Univ Sci & Technol, Coll Engn, UTS SUStech Joint Res Ctr Biomed Mat & Devices, Dept Biomed Engn, Shenzhen, Guangdong, Peoples R China

[3] Peking Univ, Coll Engn, Dept Biomed Engn, Beijing, Peoples R China

[4] Tsinghua Univ, Inst Brain & Cognit Sci, Beijing, Peoples R China

来源：

OPTICS EXPRESS | 2020年 / 28卷 / 17期

基金：

中国国家自然科学基金;

关键词：

FLUORESCENCE MICROSCOPY; LIVE CELLS; ORIENTATION; RESOLUTION; OPTIMIZATION; MODULATION; PHASE;

D O I：

10.1364/OE.395092

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Structured illumination microscopy (SIM) requires polarization control to guarantee the high-contrast illumination pattern. However, this modulated polarization will induce artifacts in SIM when imaging fluorescent dipoles. Here we proposed the polarization weighted recombination of frequency components to reconstruct SIM data with suppressed artifacts and better resolving power. Both the simulation results and experimental data demonstrate that our algorithm can obtain isotropic resolution on dipoles and resolve a clearer structure in high-density sections compared to the conventional algorithm. Our work reinforces the SIM theory and paves the avenue for the application of SIM on a polarized specimen. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：25642 / 25654

页数：13

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