A novel electrochemical aptasensor for highly sensitive and quantitative detection of the streptomycin antibiotic

In the present study, we report a facile approach to employ gold nanoparticle (AuNPs) and thiol graphene quantum dots (GQD-SH) as the nanomaterial for ultrasensitive detection of streptomycin (STR). Based on this strategy, a GQD-SH was immobilized onto the surface of a glassy carbon electrode (GCE). AuNPs have been immobilized on SH groups of GQDs through bonding formation of Au-S and Apt have been loaded on the electrode surface through the interaction between thiol group of aptamer. By incubating STR as a target onto the surface of the prepared Apt/AuNPs/GQD-SH/GCE as a proposed nanoaptasensor, the Apt/STR complex was formed and the changes of the electrochemical signal were evaluated with the EIS technique. The proposed nanoaptasensor showed wide linear range from 0.1 to 700 pg ml(-1). Finally, the proposed nanoaptasensor was successfully applied for the determination of STR in real samples and satisfactory results were obtained. (C) 2017 Published by Elsevier B.V.