Bioluminescence imaging of live infected salmonids reveals that the fin bases are the major portal of entry for Novirhabdovirus

被引:117
作者
Harmache, A
LeBerre, M
Droineau, S
Giovannini, M
Brémont, M
机构
[1] INRA, Unite Virol & Immunol Mol, F-78352 Jouy En Josas, France
[2] CEPH, INSERM, U434, Fdn Jean Dausset, F-75010 Paris, France
关键词
D O I
10.1128/JVI.80.7.3655-3659.2006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Although Novirhabdovirus viruses, like the Infectious hematopietic necrosis virus (IHNV), have been extensively studied, limited knowledge exists on the route of IHNV entry during natural infection. A recombinant IHNV (rIHNV) expressing the Renilla luciferase gene was generated and used to infect trout. A noninvasive bioluminescence assay was developed so that virus replication in live fish could be followed hours after infection. We provide here evidence that the fin bases are the portal of entry into the fish. Confirmation was brought by the use of a nonpathogenic rIHNV, which was shown to persist in fins for 3 weeks postinfection.
引用
收藏
页码:3655 / 3659
页数:5
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