Bilirubin Oxidase-Based Nanobiocathode Working in Serum-Mimic Buffer for Implantable Biofuel Cell

被引：15

作者：

Gutierrez-Sanchez, Cristina ^{[1
]}

Pita, Marcos ^{[1
]}

Toscano, Miguel D. ^{[2
]}

De Lacey, Antonio L. ^{[1
]}

机构：

[1] CSIC, Inst Catalisis & Petroleoquim, Madrid 28049, Spain

[2] Novozymes AS, Dept Prot Divers, DK-2880 Bagsvaerd, Denmark

来源：

ELECTROANALYSIS | 2013年 / 25卷 / 06期

关键词：

Bilirubin oxidase; Biofuel cell; Oxygen cathode; Gold nanoparticles; DIRECT ELECTRON-TRANSFER; BACILLUS-PUMILUS; GOLD; LACCASE; NANOPARTICLES; REDUCTION; CATHODE;

D O I：

10.1002/elan.201200668

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Low-density graphite electrodes have been covalently modified with gold nanoparticles and used as scaffold for the oriented immobilization of Myrothecium verrucaria bilirubin oxidase. The developed nanobioelectrodes have been tested as a biocathode in a human-serum surrogate buffer that mimics physiological conditions. The nanostructured bioelectrode offered a -140 mu Acm-2 current density under serum-mimic conditions at 0.2V, and was operational during at least 6days.

引用

页码：1359 / 1362

页数：4

共 22 条

[1] Enzymatic biofuel cells for Implantable and microscale devices [J].

Barton, SC ;

Gallaway, J ;

Atanassov, P .

CHEMICAL REVIEWS, 2004, 104 (10) :4867-4886

[2] Redox potentials of the blue copper sites of bilirubin oxidases [J].

Christenson, Andreas ;

Shleev, Sergey ;

Mano, Nicolas ;

Heller, Adam ;

Gorton, Lo .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2006, 1757 (12) :1634-1641

[3] An innovative powerful and mediatorless H2/O2 biofuel cell based on an outstanding bioanode [J].

Ciaccafava, A. ;

De Poulpiquet, A. ;

Techer, V. ;

Giudici-Orticoni, M. T. ;

Tingry, S. ;

Innocent, C. ;

Lojou, E. .

ELECTROCHEMISTRY COMMUNICATIONS, 2012, 23 :25-28

[4] Mechanistic studies of the 'blue' Cu enzyme, bilirubin oxidase, as a highly efficient electrocatalyst for the oxygen reduction reaction [J].

dos Santos, Luciano ;

Climent, Victor ;

Blanford, Christopher F. ;

Armstrong, Fraser A. .

PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2010, 12 (42) :13962-13974

[5] Bilirubin oxidase from Bacillus pumilus: A promising enzyme for the elaboration of efficient cathodes in biofuel cells [J].

Durand, Fabien ;

Kjaergaard, Christian Hauge ;

Suraniti, Emmanuel ;

Gounel, Sebastien ;

Hadt, Ryan G. ;

Solomon, Edward I. ;

Mano, Nicolas .

BIOSENSORS & BIOELECTRONICS, 2012, 35 (01) :140-146

[6] A highly efficient O2 cathode based on bilirubin oxidase from Bacillus pumilus operating in serum [J].

Edembe, Lise ;

Gounel, Sebastien ;

Cadet, Marine ;

Durand, Fabien ;

Mano, Nicolas .

ELECTROCHEMISTRY COMMUNICATIONS, 2012, 23 :80-82

[7] Gold Nanoparticles as Electronic Bridges for Laccase-Based Biocathodes [J].

Gutierrez-Sanchez, Cristina ;

Pita, Marcos ;

Vaz-Dominguez, Cristina ;

Shleev, Sergey ;

De Lacey, Antonio L. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (41) :17212-17220

[8] Potentially implantable miniature batteries [J].

Heller, A .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2006, 385 (03) :469-473

[9] Deactivation of bilirubin oxidase by a product of the reaction of urate and O2 [J].

Kang, C ;

Shin, H ;

Zhang, YC ;

Heller, A .

BIOELECTROCHEMISTRY, 2004, 65 (01) :83-88

[10] On the stability of the "wired" bilirubin oxidase oxygen cathode in serum [J].

Kang, C ;

Shin, H ;

Heller, A .

BIOELECTROCHEMISTRY, 2006, 68 (01) :22-26

← 1 2 3 →