HPV16/18 E5, a promising candidate for cervical cancer vaccines, affects SCPs, cell proliferation and cell cycle, and forms a potential network with E6 and E7

被引：16

作者：

Liao, Shujie ^{[1
]}

Deng, Dongrui ^{[1
]}

Hu, Xiaoji ^{[1
]}

Wang, Wei ^{[1
]}

Li, Li ^{[1
]}

Li, Wei ^{[1
]}

Zhou, Jianfeng ^{[1
]}

Xu, Gang ^{[1
]}

Meng, Li ^{[1
]}

Wang, Shixuan ^{[1
]}

Ma, Ding ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Canc Biol Res Ctr, Wuhan 430030, Hubei, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2013年 / 31卷 / 01期

基金：

美国国家科学基金会;

关键词：

human papillomavirus; E5; cervical cancer; GENE-EXPRESSION; HPV-16; E5; PROTEIN; ONCOPROTEIN; PROGRESSION; INHIBITION; ADHESION; RECEPTOR;

D O I：

10.3892/ijmm.2012.1168

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The spindle checkpoint proteins (SCPs), which sense the existence of misaligned sister chromatids during mitosis and meiosis, are essential for cell proliferation and differentiation. Therefore, the role of SCPs in carcinogenesis is gaining. increased attention. In this study, we analysed the expression of Bub1 and Mad2 in clinical samples by immunohistochemistry (IHC) during the development of cervical cancer (CC), and we explored the interaction of Bub1/Mad2 with different proteins through immunoprecipitation (IP). Furthermore, we analysed the characteristics of four different cell models of human papillomavirus (HPV)16/18 E5. We demonstrated that with the progression of CC, the expression of Bub1 and Mad2 was gradually reduced under the influence of HPVE5. Overexpression of HPV16/18 E5 significantly increased cell proliferation, as well as the percentage of cells in the S phase. In addition, the levels of p21, Bub1 and Mad2 were markedly decreased in E5-expressing cells. Therefore, HPV16/18 E5 plays a critical role in carcinogenesis and is a potential therapeutic target in CC treatment.

引用

页码：120 / 128

页数：9

共 26 条

[1] Detection of human papillomavirus type 16 early-gene transcription by reverse transcription-PCR is associated with abnormal cervical cytology [J].