Improvement of γ-aminobutyric acid (GABA) production using cell entrapment of Lactobacillus brevis GABA 057

被引：0

作者：

Choi, SI

Lee, JW

Park, SM

Lee, MY

Ji, GE

Park, MS

Heo, TR ^{[1
]}

机构：

[1] Inha Univ, Dept Biol Engn, Inchon 402751, South Korea

[2] Seoul Natl Univ, Dept Food & Nutr, Seoul 151742, South Korea

[3] BIFIDO Co Ltd, Res Ctr, Seoul 151818, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2006年 / 16卷 / 04期

关键词：

gamma-aminobutyric acid (GABA); entrapment; Lactobacillus brevis GABA 057; monosodium glutamate (MSG);

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

GABA (gamma-aminobutyric acid) is the principal inhibitory neurotransmitter in the brain. For the efficient production of GABA, a semicontinuous cell entrapment system using Lactobacillus brevis GABA 057 was optimized, including the Substrate concentration, bead-stabilizing additives, and reaction conditions. The converted monosodium glutamate (MSG), which was added as a substrate for glutamic acid decarboxylase (GAD), increased from 2% (w/v) to 12% (w/v). The addition of isomaltooligosaccharide to the alginate beads also increased the stability of the entrapped L. brevis and GABA productivity. Consequently, when optimal conditions were applied, Lip to 223 mM GABA could be produced from 534 mM MSG after 48 h of reaction by using alginate-bead-encapsulated L. brevis GABA 057.

引用

页码：562 / 568

页数：7