Nanobioprobe mediated DNA aptamers for explosive detection

被引：23

作者：

Priyanka ^{[1
,2
]}

Shorie, Munish ^{[1
]}

Bhalla, Vijayender ^{[1
]}

Pathania, Preeti ^{[1
]}

Suri, C. Raman ^{[1
]}

机构：

[1] Inst Microbial Technol CSIR, Chandigarh 160036, India

[2] Inst Nano Sci & Technol, Mohali 160064, India

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 09期

关键词：

SELECTION; SURFACE; CELLS; ANTIBODIES; EVOLUTION; TNT;

D O I：

10.1039/c3cc47562j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Specific nucleic acid aptamers using the microtiter plate based modified SELEX method against explosive trinitrotoluene are reported. Efficient partitioning of dsDNA was carried out using streptavidin labeled gold nanoprobes for the selection of specific aptamers. The selected binders having an affinity of similar to 10(-7) M were used in the newly developed electrochemical aptasensor, exhibiting a detection limit of around 1 ppb for trinitrotoluene.

引用

页码：1080 / 1082

页数：3

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