Cucumber mosaic virus in globe artichoke in Turkey

被引：4

作者：

Ergun, Muge ^{[1
]}

Erkan, Semih ^{[1
]}

Paylan, Ismail Can ^{[1
]}

机构：

[1] Ege Univ, Fac Agr, Dept Plant Protect, TR-35100 Izmir, Turkey

来源：

CANADIAN JOURNAL OF PLANT PATHOLOGY | 2013年 / 35卷 / 04期

关键词：

artichoke; Cucumber mosaic virus; DAS-ELISA; RT-PCR; virus detection;

D O I：

10.1080/07060661.2013.833987

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A survey was conducted to identify viruses infecting globe artichoke (Cynara cardunculus L. subsp. scolymus (L.) Hayek) in the most important globe artichoke-producing areas of Turkey. A total of 120 globe artichoke samples were collected from three provinces of the Aegean region in western Turkey. Samples were tested by DAS-ELISA, RT-PCR and mechanical inoculation onto indicator plants. Cucumber mosaic virus (CMV) was detected in five of the samples tested. Tomato spotted wilt virus (TSWV) and Tobacco mosaic virus (TMV) were not detected in the remaining 115 samples. In RT-PCR, amplicons of the expected size (about 540 bp) were obtained from symptomatic plants that were CMV-positive in DAS-ELISA. The sequenced 5 PCR products covering the partial CP gene revealed 99% identities at the nucleotide level with those CMV isolates. When inoculated with sap from symptomatic artichoke leaves, selected test plants developed the symptoms that are typical of CMV infection. This is the first record of CMV infecting globe artichoke in Turkey.

引用

页码：514 / 517

页数：4

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