Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations

被引：13
|
作者：
Grieshaber, Dorothee [1 ]
de Lange, Victoria [1 ]
Hirt, Thomas [2 ]
Lu, Zhihua [2 ]
Voeroes, Janos [1 ]
机构：
[1] Swiss Fed Inst Technol, Lab Biosensors & Bioelect, Inst Biomed Engn, CH-8092 Zurich, Switzerland
[2] BioCure Inc, Norcross, GA 30071 USA
来源：
SENSORS | 2008年 / 8卷 / 12期
关键词：
High sensitivity; antigen detection; biosensor; lipid vesicles; quartz crystal microbalance;
D O I：
10.3390/s8127894
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a sandwich assay by coupling the secondary antibodies to vesicles. This resulted in an increase of detection sensitivity, achieving a diagnostically relevant detection limit of 5 ng/ml or 30 pM antigens. In addition, we could combine the individual assay steps to decrease the total time to result in about 30 minutes.
引用
收藏
页码：7894 / 7903
页数：10
相关论文
共 50 条
  • [41] Dual Signal Amplification Electrochemical Biosensor for Lead Cation
    Zhang, Jing
    Lin, Yan
    Peng, Hong
    Hong, Nian
    Cheng, Lin
    Wei, Guobing
    Fan, Hao
    ELECTROANALYSIS, 2018, 30 (05) : 955 - 961
  • [42] Controlled-Release Electrochemiluminescence Biosensor with Strong Self-On Effect by a Multiple Signal Amplification Strategy for Trace Detection of Prostate-Specific Antigen
    Huang, Ziqiu
    Zhao, Lu
    Guo, Qingfang
    Fan, Dawei
    Ren, Xiang
    Wei, Qin
    Wu, Dan
    ANALYTICAL CHEMISTRY, 2024, 96 (17) : 6659 - 6665
  • [43] An enzyme-free biosensor for sensitive detection of Salmonella using curcumin as signal reporter and click chemistry for signal amplification
    Huang, Fengchun
    Xue, Li
    Zhang, Huilin
    Guo, Ruya
    Li, Yanbin
    Liao, Ming
    Wang, Maohua
    Lin, Jianhan
    THERANOSTICS, 2018, 8 (22): : 6263 - 6273
  • [44] MEMS BASED IMPEDANCE BIOSENSOR FOR RAPID DETECTION OF LOW CONCENTRATIONS OF FOODBORNE PATHOGENS
    Abdullah, Amjed
    Jasim, Ibrahim
    Alalem, Mohammed
    Dweik, Majed
    Almasri, Mahmoud
    30TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2017), 2017, : 381 - 384
  • [45] Surface modification of an acoustic biosensor allowing the detection of low concentrations of cancer markers
    Gruhl, Friederike J.
    Laenge, Kerstin
    ANALYTICAL BIOCHEMISTRY, 2012, 420 (02) : 188 - 190
  • [46] Electrochemical immunoassay for procalcitonin antigen detection based on signal amplification strategy of multiple nanocomposites
    Fang, Yi-Shan
    Wang, Hai-Ying
    Wang, Li-Shi
    Wang, Ju-Fang
    BIOSENSORS & BIOELECTRONICS, 2014, 51 : 310 - 316
  • [47] Signal amplification strategies in biosensing of extracellular vesicles (EVs)
    Khaksari, Sedighe
    Abnous, Khalil
    Hadizadeh, Farzin
    Ramezani, Mohammad
    Taghdisi, Seyed Mohammad
    Shaegh, Seyed Ali Mousavi
    TALANTA, 2023, 256
  • [48] Optimizing immunohistochemistry: antigen retrieval and signal amplification
    McNicol, AM
    Richmond, JA
    HISTOPATHOLOGY, 1998, 32 (02) : 97 - 103
  • [49] A low-noise ratiometric fluorescence biosensor for detection of Pb2+ based on DNAzyme and exonuclease III–assisted cascade signal amplification
    Huali Jin
    Ruike Liu
    Tian Bai
    Min Wei
    Baoshan He
    Zhiguang Suo
    Analytical and Bioanalytical Chemistry, 2022, 414 : 1899 - 1907
  • [50] Antigen retrieval, signal amplification and intensification in immunohistochemistry
    Werner, M
    vonWasielewski, R
    Komminoth, P
    HISTOCHEMISTRY AND CELL BIOLOGY, 1996, 105 (04) : 253 - 260
12345