Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations

被引：13
|
作者：
Grieshaber, Dorothee [1 ]
de Lange, Victoria [1 ]
Hirt, Thomas [2 ]
Lu, Zhihua [2 ]
Voeroes, Janos [1 ]
机构：
[1] Swiss Fed Inst Technol, Lab Biosensors & Bioelect, Inst Biomed Engn, CH-8092 Zurich, Switzerland
[2] BioCure Inc, Norcross, GA 30071 USA
来源：
SENSORS | 2008年 / 8卷 / 12期
关键词：
High sensitivity; antigen detection; biosensor; lipid vesicles; quartz crystal microbalance;
D O I：
10.3390/s8127894
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a sandwich assay by coupling the secondary antibodies to vesicles. This resulted in an increase of detection sensitivity, achieving a diagnostically relevant detection limit of 5 ng/ml or 30 pM antigens. In addition, we could combine the individual assay steps to decrease the total time to result in about 30 minutes.
引用
收藏
页码：7894 / 7903
页数：10
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