Genetic Engineering as a Strategy to Improve the Therapeutic Efficacy of Mesenchymal Stem/Stromal Cells in Regenerative Medicine

被引：78

作者：

Damasceno, Patricia Kauanna Fonseca ^{[1
,2
]}

de Santana, Thais Alves ^{[1
]}

Santos, Girlaine Cafe ^{[1
]}

Orge, Iasmim Diniz ^{[1
,2
]}

Silva, Daniela Nascimento ^{[1
,2
]}

Albuquerque, Juliana Fonseca ^{[1
]}

Golinelli, Giulia ^{[3
]}

Grisendi, Giulia ^{[3
]}

Pinelli, Massimo ^{[4
]}

Ribeiro dos Santos, Ricardo ^{[1
,2
,5
]}

Dominici, Massimo ^{[3
]}

Soares, Milena Botelho Pereira ^{[1
,2
,5
]}

机构：

[1] Fiocruz MS, Oswaldo Cruz Fdn, Goncalo Moniz Inst, Salvador, BA, Brazil

[2] SENAI CIMATEC, Hlth Inst Technol, Salvador, BA, Brazil

[3] Univ Modena & Reggio Emilia, Div Oncol, Lab Cellular Therapy, Modena, Italy

[4] Univ Modena & Reggio Emilia, Dept Med & Surg Sci Children & Adults, Div Plast Surg, Modena, Italy

[5] Natl Inst Sci & Technol Regenerat Med INCT REGENE, Rio De Janeiro, Brazil

来源：

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | 2020年 / 8卷

关键词：

mesenchymal stem; stromal cells; genetic engineering; regenerative medicine; cell therapy; gene therapy; MARROW STROMAL CELLS; ACUTE LUNG INJURY; OVEREXPRESSING HEME OXYGENASE-1; NEURAL STEM-CELLS; BONE-MARROW; SPINAL-CORD; RAT MODEL; MYOCARDIAL-INFARCTION; CARDIAC-FUNCTION; FUNCTIONAL RECOVERY;

D O I：

10.3389/fcell.2020.00737

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Mesenchymal stem/stromal cells (MSCs) have been widely studied in the field of regenerative medicine for applications in the treatment of several disease settings. The therapeutic potential of MSCs has been evaluated in studiesin vitroandin vivo, especially based on their anti-inflammatory and pro-regenerative action, through the secretion of soluble mediators. In many cases, however, insufficient engraftment and limited beneficial effects of MSCs indicate the need of approaches to enhance their survival, migration and therapeutic potential. Genetic engineering emerges as a means to induce the expression of different proteins and soluble factors with a wide range of applications, such as growth factors, cytokines, chemokines, transcription factors, enzymes and microRNAs. Distinct strategies have been applied to induce genetic modifications with the goal to enhance the potential of MCSs. This review aims to contribute to the update of the different genetically engineered tools employed for MSCs modification, as well as the factors investigated in different fields in which genetically engineered MSCs have been tested.

引用

页数：24

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