Lipid bilayer array for simultaneous recording of ion channel activities

被引：11

作者：

Hirano-Iwata, Ayumi ^{[1
,2
]}

Nasu, Tomohiro ^{[1
]}

Oshima, Azusa ^{[1
]}

Kimura, Yasuo ^{[3
]}

Niwano, Michio ^{[1
,3
]}

机构：

[1] Tohoku Univ, Grad Sch Biomed Engn, Aoba Ku, Sendai, Miyagi 9808579, Japan

[2] Japan Sci & Technol Agcy JST, PRESTO, Kawaguchi, Saitama 3320012, Japan

[3] Tohoku Univ, Elect Commun Res Inst, Lab Nanoelect & Spintron, Sendai, Miyagi 9808577, Japan

来源：

APPLIED PHYSICS LETTERS | 2012年 / 101卷 / 02期

基金：

日本学术振兴会;

关键词：

RECONSTITUTION; MEMBRANES; GRAMICIDIN;

D O I：

10.1063/1.4736263

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

This paper describes an array of stable and reduced-solvent bilayer lipid membranes (BLMs) formed in microfabricated silicon chips. BLMs were first vertically formed simultaneously and then turned 90 degrees in order to realize a horizontal BLM array. Since the present BLMs are mechanically stable and robust, the BLMs survive this relatively tough process. Typically, a similar to 60% yield in simultaneous BLM formation over 9 sites was obtained. Parallel recordings of gramicidin channel activities from different BLMs were demonstrated. The present system has great potential as a platform of BLM-based high throughput drug screening for ion channel proteins. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4736263]

引用

页数：3

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