Evaluation of genetic damage in a Brazilian population occupationally exposed to pesticides and its correlation with polymorphisms in metabolizing genes

被引：91

作者：

da Silva, Juliana ^{[1
]}

Moraes, Camila R. ^{[2
]}

Heuser, Vanina D. ^{[3
,4
]}

Andrade, Vanessa M. ^{[3
,5
]}

Silva, Fernanda R. ^{[3
]}

Kvitko, Katia ^{[3
]}

Emmel, Vanessa ^{[3
]}

Rohr, Paula ^{[3
]}

Bordin, Diana Lilian ^{[6
]}

Andreazza, Ana Cristina ^{[6
]}

Salvador, Mirian ^{[6
]}

Henriques, Joao A. P. ^{[1
,2
,3
,6
]}

Erdtmann, Bernardo ^{[6
]}

机构：

[1] Univ Luterana Brasil, Lab Genet Toxicol PPGGTA & PPGECIM, Canoas, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Dept Biofis, Programa Pos Grad Biol Celular & Mol, Porto Alegre, RS, Brazil

[3] Univ Fed Rio Grande do Sul, Dept Genet, Programa Pos Grad Genet & Biol Mol, Porto Alegre, RS, Brazil

[4] Karolinska Inst, Inst Environm Med, Div Biochem Toxicol, S-10401 Stockholm, Sweden

[5] Univ Extremo Sul Catarinense, Programa Pos Grad Ciencias Saude, Lab Imunol & Mutagenese, Criciuma, SC, Brazil

[6] Univ Caxias Sul, Inst Biotecnol, Caxias Do Sul, RS, Brazil

来源：

MUTAGENESIS | 2008年 / 23卷 / 05期

关键词：

D O I：

10.1093/mutage/gen031

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Cytogenetic damage in individuals occupationally exposed to pesticides has received the attention of investigators in several countries, but no definitive conclusions can yet be made. The present study aimed at assessing if prolonged exposure to complex mixtures of pesticides leads to an increase in cytogenetic damage. Vineyard workers exposed to pesticides in Caxias do Sul (Brazil) were evaluated using the micronucleus (MN) test in binucleated lymphocytes and the comet assay in peripheral leukocytes. In order to evaluate if genetically determined individual variations in xenobiotic metabolizing capacity could modify individual susceptibility to the possible genotoxic effects of pesticides, the subjects were genotyped for several genes: GSTT1, GSTM1, GSTP1, CYP1A1, CYP2E1 and PON. The study involved a total number of 173 men: 108 were agricultural workers exposed to pesticides and 65 were controls. The present study showed a high rate of MN and DNA damage in pesticide-exposed individuals (P <= 0.001; Mann-Whitney U-test). In addition, some effects of genetic polymorphisms in PON in the modulation of MN results were observed in the exposed group, and an association between GSTM1, GSTT1 and CYP2E1 polymorphisms was suggested.

引用

页码：415 / 422

页数：8

共 61 条

[1]

AMORIM LMF, 2002, CANC LETT, V181, P179

[2]

[Anonymous], INT RISK INF IRIS

[3] An exploratory analysis of the effect of pesticide exposure on the risk of spontaneous abortion in an Ontario farm population [J].

Arbuckle, TE ;

Lin, ZQ ;

Mery, LS .

ENVIRONMENTAL HEALTH PERSPECTIVES, 2001, 109 (08) :851-857

[4]

Au WW, 1999, ENVIRON HEALTH PERSP, V107, P501

[5] Factors contributing to discrepancies in population monitoring studies [J].

Au, WW ;

Cajas-Salazar, N ;

Salama, S .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1998, 400 (1-2) :467-478

[6] GENETIC RISK AND CARCINOGEN EXPOSURE - A COMMON INHERITED DEFECT OF THE CARCINOGEN-METABOLISM GENE GLUTATHIONE-S-TRANSFERASE M1 (GSTM1) THAT INCREASES SUSCEPTIBILITY TO BLADDER-CANCER [J].

BELL, DA ;

TAYLOR, JA ;

PAULSON, DF ;

ROBERTSON, CN ;

MOHLER, JL ;

LUCIER, GW .

JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1993, 85 (14) :1159-1164

[7]

BLAIR A, 1983, J NATL CANCER I, V71, P31

[8] Genotoxicity of pesticides: a review of human biomonitoring studies [J].

Bolognesi, C .

MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH, 2003, 543 (03) :251-272

[9] Evidence for genotoxicity of pesticides in pesticide applicators: a review [J].

Bull, S ;

Fletcher, K ;

Boobis, AR ;

Battershill, JM .

MUTAGENESIS, 2006, 21 (02) :93-103

[10] CONSIDERATIONS FOR POPULATION MONITORING USING CYTOGENETIC TECHNIQUES [J].

CARRANO, AV ;

NATARAJAN, AT .

MUTATION RESEARCH, 1988, 204 (03) :379-406

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