Sex Steroid Hormone Analysis in Human Tear Fluid Using a Liquid Chromatography-Mass Spectrometry Method

被引:5
作者
Robciuc, Alexandra [1 ,2 ]
Savolainen-Peltonen, Hanna [1 ]
Haanpaa, Mikko [3 ]
Moilanen, Jukka A. O. [2 ]
Mikkola, Tomi S. [1 ]
机构
[1] Univ Helsinki, Helsinki Univ Hosp, Obstet & Gynecol, FIN-00029 Hus Helsinki, Finland
[2] Univ Helsinki, Helsinki Univ Hosp, Ophthalmol Dept, Helsinki Eye Lab, FIN-00029 Hus Helsinki, Finland
[3] Helsinki Univ Hosp, HUSLAB, FIN-00029 Hus Helsinki, Finland
基金
芬兰科学院;
关键词
tear fluid; steroid hormones; estrogen; testosterone; mass spectrometry; high performance liquid chromatography; DRY EYE DISEASE; MESSENGER-RNAS; IDENTIFICATION; ESTROGEN; RECEPTOR; ANDROGEN; PROTEIN;
D O I
10.3390/ijms232314864
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The marked sexual dimorphism prevalent in inflammatory/autoimmune diseases is mostly due to sex hormone actions. One common eye disease that disproportionately affects women is dry eye. Thus, our aim was to optimise our highly sensitive liquid chromatography-tandem mass spectrometry method for steroid hormone quantification in tear fluid (TF). We used tears and matched serum samples from 10 heathy individuals. Estrone, estradiol testosterone, progesterone, androstenedione, and dehydroepiandrosterone, were quantified with an HPLC coupled with a Triple Quad 5500 MS. Estrone was measured in 80% of female and 20% of male TF samples (mean +/- SD, 68.9 +/- 62.2 pmol/L), whereas estradiol was undetectable in tears. Progesterone was identified in half of the female tear samples (2.91 +/- 3.47 nmol/L) but in none of the male samples, whereas testosterone was quantifiable only in male tears (0.24 +/- 0.1 nmol/L). TF hormone levels were, on average, from 1.4% to 55% of systemic values. Estrone, progesterone, and testosterone levels in tears correlated with the matching serum samples (r = 0.82, 0.79, and 0.85, respectively), but androstenedione and dehydroepiandrosterone showed no correlations. Our LC-MS/MS method could detect five out of the six steroid hormones studied in individual human TF samples and could therefore be used to analyse the role of sex steroids in eye diseases.
引用
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页数:8
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