Light and sugar regulation of the barley sucrose: fructan 6-fructosyltransferase promoter

被引：37

作者：

Nagaraj, VJ ^{[1
]}

Riedl, R ^{[1
]}

Boller, T ^{[1
]}

Wiemken, A ^{[1
]}

Meyer, AD ^{[1
]}

机构：

[1] Univ Basel, Inst Bot, CH-4056 Basel, Switzerland

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2001年 / 158卷 / 12期

关键词：

fructan; Hordeum vulgare; carbohydrate metabolism; promoter; sucrose : fructan 6-fructosyltransferase; light-regulation; sugar-sensing;

D O I：

10.1078/0176-1617-00592

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Fructans are a class of major storage compounds in many plants including several economically important cereals. Earlier we characterized and cloned the sucrose: fructan 6-fructosyltransferase (6-SFT) of barley (Hordeum vulgare L), a key enzyme for the synthesis of branched fructans (graminans) typical of many cereals and temperate forage grasses. Here, we describe the cloning of the barley 6-SFT promoter region, by PCR-based genome walking procedures. Using a promoter-reporter gene construct (uidA encoding P-glucuronidase) and microprojectile bombardment of excised barley leaves, we show that the cloned sequence contains the necessary cis acting elements conferring sucrose and light induction of 6-SFT transcription.

引用

页码：1601 / 1607

页数：7