Chemical genetics: Drug screens in zebrafish

被引:72
作者
den Hertog, J [1 ]
机构
[1] Netherlands Inst Dev Biol, Hubrecht Lab, NL-3584 CT Utrecht, Netherlands
关键词
chemical genetics; zebrafish; development; compounds; inhibitors; drug target; disease;
D O I
10.1007/s10540-005-2891-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
High throughput chemical genetic screens for compounds with specific biological activity in a whole organism are feasible using zebrafish embryos. At least two medium to large scale drug screens have been carried out to date, leading to the identification of compounds that disturb zebrafish development. Chemical genetics using zebrafish embryos may become an important step in the discovery of drugs and their targets.
引用
收藏
页码:289 / 297
页数:9
相关论文
共 22 条
  • [1] Innovation - New tools for functional mammalian cancer genetics
    Brummelkamp, TR
    Bernards, R
    [J]. NATURE REVIEWS CANCER, 2003, 3 (10) : 781 - 789
  • [2] Dissection of angiogenic signaling in zebrafish using a chemical genetic approach
    Chan, J
    Bayliss, PE
    Wood, JM
    Roberts, TM
    [J]. CANCER CELL, 2002, 1 (03) : 257 - 267
  • [3] Zebrafish: bridging the gap between development and disease
    Dodd, A
    Curtis, PM
    Williams, LC
    Love, DA
    [J]. HUMAN MOLECULAR GENETICS, 2000, 9 (16) : 2443 - 2449
  • [4] Short interfering RNA-mediated gene targeting in the zebrafish
    Dodd, A
    Chambers, SP
    Love, DR
    [J]. FEBS LETTERS, 2004, 561 (1-3) : 89 - 93
  • [5] Inhibition of zebrafish fgf8 Pre-mRNA splicing with morpholino oligos:: A quantifiable method for gene knockdown
    Draper, BW
    Morcos, PA
    Kimmel, CB
    [J]. GENESIS, 2001, 30 (03) : 154 - 156
  • [6] Driever W, 1996, DEVELOPMENT, V123, P37
  • [7] Morphant technology in model developmental systems
    Ekker, SC
    Larson, JD
    [J]. GENESIS, 2001, 30 (03) : 89 - 93
  • [8] Insertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate development
    Golling, G
    Amsterdam, A
    Sun, ZX
    Antonelli, M
    Maldonado, E
    Chen, WB
    Burgess, S
    Haldi, M
    Artzt, K
    Farrington, S
    Lin, SY
    Nissen, RM
    Hopkins, N
    [J]. NATURE GENETICS, 2002, 31 (02) : 135 - 140
  • [9] Analysis of a zebrafish VEGF receptor mutant reveals specific disruption of angiogenesis
    Habeck, H
    Odenthal, J
    Walderich, B
    Maischien, HM
    Schulte-Merker, S
    [J]. CURRENT BIOLOGY, 2002, 12 (16) : 1405 - 1412
  • [10] Haffter P, 1996, DEVELOPMENT, V123, P1