Circulating microRNA expression profiles to identify a potential link between prostate cancer and obesity

被引:2
|
作者
Arisan, Serdar [1 ]
Ozfiliz-Kilbas, Pelin [2 ]
Rencuzogullari, Ozge [2 ]
Unsal, Narcin Palavan [3 ]
Coker-Gurkan, Ajda [4 ]
Obakan-Yerlikaya, Pinar [5 ,6 ]
Uysal-Onganer, Pinar [7 ]
Arisan, Elif Damla [8 ]
机构
[1] Saglik Bilimleri Univ, Hamidiye Med Sch, Dept Urol, Istanbul, Turkey
[2] Istanbul Kultur Univ, Dept Mol Biol & Genet, Istanbul, Turkey
[3] Netkent Mediterranean Res & Sci Univ, Kyrenia, North Cyprus, Turkey
[4] Marmara Univ, Dept Biol, Istanbul, Turkey
[5] Istanbul Medeniyet Univ, Dept Mol Biol & Genet, Istanbul, Turkey
[6] Istanbul Medeniyet Univ, Sci & Adv Technol Res Ctr, Istanbul, Turkey
[7] Univ Westminster, Sch Life Sci, Canc Res Grp, London W1W 6UW 3, England
[8] Gebze Tech Univ, Inst Biotechnol, Kocaeli, Turkey
来源
HUMAN GENE | 2022年 / 34卷
关键词
Prostate cancer; Obesity; miR-100; miR-141; miR-145; miR-4463; miR-653; INSULIN SENSITIVITY; ADIPONECTIN; RECURRENCE; OVERWEIGHT; PREDICTION; RESISTANCE; THERAPY; TARGETS; MIRNA;
D O I
10.1016/j.humgen.2022.201109
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Effective diagnostic methods are needed to apply appropriate treatment strategies in patients with aggressive prostate cancer. From this point of view, risk factors that cause prostate cancer or its aggressiveness should be considered. Obesity is a critical risk factor for triggering prostate cancer's metastatic properties. microRNAs are used as biomarkers in diagnosing cancer and obesity depending on their tissue-specific expression patterns. This study investigates the role of obesity in the metastatic profile of prostate cancer depending on the differential expression signatures of selected miRNAs in prostate cancer and obese patients. The roles of miR-100, miR-141 and miR-145 in prostate cancer and obesity are partially known. However, their potential to become circular biomarkers in the blood is not elucidated. There is no previous data on miR-4463 and miR-653 on prostate cancer and obesity association. In this study, the blood samples were taken and obtained serum from 69 patients of 6 subgroups that consisted of one healthy group and five unhealthy groups based on their different prostate cancer or obesity levels. Five selected miRNA expression analyses (miR-100, miR-141, miR-145, miR-4463, and miR-653) were performed through total RNA isolation, which was confirmed via synthetic cel-miR-39 miRNA. Quantitative Real-Time PCR analyzed the expression levels of selected miRNAs. Data analysis was performed via normalising target miRNA expression levels with cel-miR-39. In this study, we found that the relationship between prostate cancer and obesity was investigated at the molecular level. It was suggested that target miR-100 could be a promising biomarker for non-obese and aggressive prostate cancer patients. miR-145 is a more potential biomarker than miR-141 for non-aggressive and non-obese patients. miR-4463 can be used to predict more prostate cancer patients than obese patients. Lastly, miR-653 can be a biomarker for non-aggressive prostate cancer cells.
引用
收藏
页数:11
相关论文
共 50 条
  • [1] Circulating microRNA expression profiles in ovarian cancer
    Ayaz, L.
    Cayan, F.
    Balci, S.
    Gorur, A.
    Akbayir, S.
    Yaroglu, H. Yildirim
    Unal, N. Dogruer
    Tamer, L.
    JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 2014, 34 (07) : 620 - 624
  • [2] Missing link between microRNA and prostate cancer
    Gill, Balraj Singh
    Alex, Jimi Marin
    Navgeet
    Kumar, Sanjeev
    TUMOR BIOLOGY, 2016, 37 (05) : 5683 - 5704
  • [3] Gene expression profiling of prostate tissue identifies chromatin regulation as a potential link between obesity and lethal prostate cancer
    Ebot, Ericka M.
    Gerke, Travis
    Labbe, David P.
    Sinnott, Jennifer A.
    Zadra, Giorgia
    Rider, Jennifer R.
    Tyekucheva, Svitlana
    Wilson, Kathryn M.
    Kelly, Rachel S.
    Shui, Irene M.
    Loda, Massimo
    Kantoff, Philip W.
    Finn, Stephen
    Heiden, Matthew G. Vander
    Brown, Myles
    Giovannucci, Edward L.
    Mucci, Lorelei A.
    CANCER, 2017, 123 (21) : 4130 - 4138
  • [4] Expression of microRNAs: potential molecular link between obesity, diabetes and cancer
    Ali, A. S.
    Ali, S.
    Ahmad, A.
    Bao, B.
    Philip, P. A.
    Sarkar, F. H.
    OBESITY REVIEWS, 2011, 12 (12) : 1050 - 1062
  • [5] MicroRNA in prostate cancer: functional importance and potential as circulating biomarkers
    Benjamin L Jackson
    Anna Grabowska
    Hari L Ratan
    BMC Cancer, 14
  • [6] MicroRNA in prostate cancer: functional importance and potential as circulating biomarkers
    Jackson, Benjamin L.
    Grabowska, Anna
    Ratan, Hari L.
    BMC CANCER, 2014, 14
  • [7] Circulating microRNA expression in connection with prostate cancer lymphogenous metastasis
    Shkurnikov, M. Yu.
    Makarova, Yu. A.
    Knyazev, E. N.
    Zotikov, A. A.
    Nyushko, K. M.
    Alekseev, B. Ya.
    Kaprin, A. D.
    ONKOUROLOGIYA, 2018, 14 (01): : 87 - 93
  • [8] Mechanistic Insights into the Link between Obesity and Prostate Cancer
    Adesunloye, Bamidele A.
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (08)
  • [9] Validation of differential expression of microRNA profiles in prostate cancer specimens
    Waingankar, Nikhil
    Broccoli, Nicholas
    Rais-Bahrami, Soroush
    Smith, Kevin
    Oswald, Michaela
    Khalili, Houman
    Lee, Annette
    Chan, Theresa
    Yaskiv, Oksana
    Gregersen, Peter
    Vira, Manish Arvind
    JOURNAL OF CLINICAL ONCOLOGY, 2014, 32 (04)
  • [10] Circulating MicroRNA Profiles as Potential Biomarkers for Differentiated Thyroid Cancer Recurrence
    Miljus, Jelena Jankovic
    Augusta Guillen-Sacoto, Maria
    Makiadi-Alvarado, Jennifer
    Wert-Lamas, Leon
    Ramirez-Moya, Julia
    Robledo, Mercedes
    Santisteban, Pilar
    Riesco-Eizaguirre, Garcilaso
    JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2022, 107 (05): : 1280 - 1293