Vesicular stomatitis virus is a negative-stranded RNA virus. Its nucleoprotein (N) binds the viral genomic RNA and is involved in multiple functions including transcription, replication, and assembly. We have determined a 2.9 angstrom structure of a complex containing 10 molecules of the N protein and 90 bases of RNA. The RNA is tightly sequestered in a cavity at the interface between two lobes of the N protein. This serves to protect the RNA in the absence of polynucleotide synthesis. For the RNA to be accessed, some conformational change in the N protein should be necessary.
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Univ E Anglia, Norwich Med Sch, Biomed Res Ctr, Norwich NR4 7TJ, Norfolk, England
Univ St Andrews, Sch Biol, St Andrews KY16 9ST, Fife, ScotlandUniv E Anglia, Norwich Med Sch, Biomed Res Ctr, Norwich NR4 7TJ, Norfolk, England
Dong, Haohao
Li, Ping
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Univ St Andrews, Sch Biol, St Andrews KY16 9ST, Fife, ScotlandUniv E Anglia, Norwich Med Sch, Biomed Res Ctr, Norwich NR4 7TJ, Norfolk, England
Li, Ping
Boettcher, Bettina
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Univ Edinburgh, Sch Biol Sci, Edinburgh EH9 3JR, Midlothian, ScotlandUniv E Anglia, Norwich Med Sch, Biomed Res Ctr, Norwich NR4 7TJ, Norfolk, England
Boettcher, Bettina
Elliott, Richard M.
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Univ St Andrews, Sch Biol, St Andrews KY16 9ST, Fife, ScotlandUniv E Anglia, Norwich Med Sch, Biomed Res Ctr, Norwich NR4 7TJ, Norfolk, England
Elliott, Richard M.
Dong, Changjiang
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Univ E Anglia, Norwich Med Sch, Biomed Res Ctr, Norwich NR4 7TJ, Norfolk, EnglandUniv E Anglia, Norwich Med Sch, Biomed Res Ctr, Norwich NR4 7TJ, Norfolk, England
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Inst Pasteur, Unite Virol Struct, Dept Virol, F-75724 Paris 15, France
CNRS, URA 3015, F-75724 Paris 15, France
Univ Paris 11, F-91405 Orsay, FranceUniv Glasgow, Ctr Virus Res, MRC, Glasgow G11 5JR, Lanark, Scotland