Structure of the vesicular stomatitis virus nucleoprotein-RNA complex

被引:263
|
作者
Green, Todd J. [1 ]
Zhang, Xin [1 ]
Wertz, Gail W. [1 ]
Luo, Ming [1 ]
机构
[1] Univ Alabama, Sch Med, Dept Microbiol, Birmingham, AL 35294 USA
关键词
D O I
10.1126/science.1126953
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Vesicular stomatitis virus is a negative-stranded RNA virus. Its nucleoprotein (N) binds the viral genomic RNA and is involved in multiple functions including transcription, replication, and assembly. We have determined a 2.9 angstrom structure of a complex containing 10 molecules of the N protein and 90 bases of RNA. The RNA is tightly sequestered in a cavity at the interface between two lobes of the N protein. This serves to protect the RNA in the absence of polynucleotide synthesis. For the RNA to be accessed, some conformational change in the N protein should be necessary.
引用
收藏
页码:357 / 360
页数:4
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