Monitoring Cellular Uptake and Cytotoxicity of Copper(II) Complex Using a Fluorescent Anthracene Thiosemicarbazone Ligand

被引：50

作者：

Kate, Anup N. ^{[1
]}

Kumbhar, Anupa A. ^{[1
]}

Khan, Ayesha A. ^{[1
]}

Joshi, Pranaya V. ^{[2
]}

Puranik, Vedavati G. ^{[2
]}

机构：

[1] Univ Pune, Dept Chem, Pune 411007, Maharashtra, India

[2] Natl Chem Lab, Ctr Mat Characterizat, Pune 411008, Maharashtra, India

来源：

BIOCONJUGATE CHEMISTRY | 2014年 / 25卷 / 01期

关键词：

RIBONUCLEOTIDE REDUCTASE INHIBITORS; DINUCLEAR PLATINUM COMPLEXES; METAL-COMPLEXES; LINKING LIGANDS; DNA CLEAVAGE; HUMAN CANCER; PHASE-I; BINDING; MECHANISM; INSIGHTS;

D O I：

10.1021/bc400385d

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The thiosemicarbazone derivative of anthracene (ATSC, anthracene thiosemicarbazone I) and its copper(II) complex (CuATSC, 2) were synthesized and characterized by spectroscopic, electrochemical, and crystallographic techniques. Interaction of 1 and 2 with calf thymus (CT) DNA was explored using absorption and emission spectral methods, and viscosity measurements reveal a partial-intercalation binding mode. Their protein binding ability was monitored by the quenching of tryptophan emission using bovine serum albumin (BSA) as a model protein. Furthermore, their cellular uptake, in vitro cytotoxicity testing on the HeLa cell line, and flow cytometric analysis were carried out to ascertain the mode of cell death. Cell cycle analysis indicated that 1 and 2 cause cell cycle arrest in sub-G1 phase.

引用

页码：102 / 114

页数：13

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