UVSSA, UBP12, and RDO2/TFIIS Contribute to Arabidopsis UV Tolerance

被引:23
作者
Al Khateeb, Wesam M. [1 ]
Sher, Annan A. [2 ]
Marcus, Jeffery M. [2 ]
Schroeder, Dana F. [2 ]
机构
[1] Yarmouk Univ, Dept Biol Sci, Irbid, Jordan
[2] Univ Manitoba, Dept Biol Sci, Winnipeg, MB, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
Arabidopsis; transcription coupled repair; UV; CSA; CSB; UVSSA; UBP7; TFIIS; COCKAYNE-SYNDROME-A; COUPLED DNA-REPAIR; GENE-EXPRESSION; EXCISION-REPAIR; TRANSCRIPT CLEAVAGE; PROTEIN; GENOME; TFIIS; COMPONENTS; INTEGRITY;
D O I
10.3389/fpls.2019.00516
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plant DNA is damaged by exposure to solar radiation, which includes ultraviolet (UV) rays. UV damaged DNA is repaired either by photolyases, using visible light energy, or by nucleotide excision repair (NER), also known as dark repair. NER consists of two subpathways: global genomic repair (GGR), which repairs untranscribed DNA throughout the genome, and transcription-coupled repair (TCR), which repairs transcribed DNA. In mammals, CSA, CSB, UVSSA, USP7, and TFIIS have been implicated in TCR. Arabidopsis homologs of CSA (AtCSA-1/2) and CSB (CHR8) have previously been shown to contribute to UV tolerance. Here we examine the role of Arabidopsis homologs of UVSSA, USP7 (UBP12/13), and TFIIS (RDO2) in UV tolerance. We find that loss of function alleles of UVSSA, UBP12, and RDO2 exhibit increased UV sensitivity in both seedlings and adults. UV sensitivity in atcsa-1, uvssa, and ubp12 mutants is specific to dark conditions, consistent with a role in NER. Interestingly, chr8 mutants exhibit UV sensitivity in both light and dark conditions, suggesting that the Arabidopsis CSB homolog may play a role in both NER and light repair. Overall our results indicate a conserved role for UVSSA, USP7 (UBP12), and TFIIS (RDO2) in TCR.
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页数:9
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共 59 条
[21]   Inhibition of UVSSA ubiquitination suppresses transcription-coupled nucleotide excision repair deficiency caused by dissociation from USP7 [J].
Higa, Mitsuru ;
Tanaka, Kiyoji ;
Saijo, Masafumi .
FEBS JOURNAL, 2018, 285 (05) :965-976
[22]   Stabilization of Ultraviolet (UV)-stimulated Scaffold Protein A by Interaction with Ubiquitin-specific Peptidase 7 Is Essential for Transcription-coupled Nucleotide Excision Repair [J].
Higa, Mitsuru ;
Zhang, Xue ;
Tanaka, Kiyoji ;
Saijo, Masafumi .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2016, 291 (26) :13771-13779
[23]   SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome [J].
Hooper, Cornelia M. ;
Tanz, Sandra K. ;
Castleden, Ian R. ;
Vacher, Michael A. ;
Small, Ian D. ;
Millar, A. Harvey .
BIOINFORMATICS, 2014, 30 (23) :3356-3364
[24]   The Translation Elongation Factor eEF-1Bβ1 Is Involved in Cell Wall Biosynthesis and Plant Development in Arabidopsis thaliana [J].
Hossain, Zakir ;
Amyot, Lisa ;
McGarvey, Brian ;
Gruber, Margaret ;
Jung, Jinwook ;
Hannoufa, Abdelali .
PLOS ONE, 2012, 7 (01)
[25]   Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR [J].
Jain, Mukesh ;
Nijhawan, Aashima ;
Tyagi, Akhilesh K. ;
Khurana, Jitendra P. .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 345 (02) :646-651
[26]   Transcription factor IIS impacts UV-inhibited transcription [J].
Jensen, Anne ;
Mullenders, Leon H. F. .
DNA REPAIR, 2010, 9 (11) :1142-1150
[27]   The Deubiquitinating Enzymes UBP12 and UBP13 Positively Regulate MYC2 Levels in Jasmonate Responses [J].
Jeong, Jin Seo ;
Jung, Choonkyun ;
Seo, Jun Sung ;
Kim, Ju-Kon ;
Chua, Nam-Hai .
PLANT CELL, 2017, 29 (06) :1406-1424
[28]   Comparative TFIIS-mediated transcript cleavage by mammalian RNA polymerase II arrested at a lesion in different transcription systems [J].
Kalogeraki, VS ;
Tornaletti, S ;
Cooper, PK ;
Hanawalt, PC .
DNA REPAIR, 2005, 4 (10) :1075-1087
[29]   The AtGenExpress global stress expression data set: protocols, evaluation and model data analysis of UV-B light, drought and cold stress responses [J].
Kilian, Joachim ;
Whitehead, Dion ;
Horak, Jakub ;
Wanke, Dierk ;
Weinl, Stefan ;
Batistic, Oliver ;
D'Angelo, Cecilia ;
Bornberg-Bauer, Erich ;
Kudla, Joerg ;
Harter, Klaus .
PLANT JOURNAL, 2007, 50 (02) :347-363
[30]   GABI-Kat SimpleSearch: new features of the Arabidopsis thaliana T-DNA mutant database [J].
Kleinboelting, Nils ;
Huep, Gunnar ;
Kloetgen, Andreas ;
Viehoever, Prisca ;
Weisshaar, Bernd .
NUCLEIC ACIDS RESEARCH, 2012, 40 (D1) :D1211-D1215