Rapid parallel flow cytometry assays of active GTPases using effector beads

被引：15

作者：

Buranda, Tione ^{[1
,2
,3
]}

BasuRay, Soumik ^{[1
]}

Swanson, Scarlett ^{[1
]}

Agola, Jacob ^{[1
]}

Bondu, Virginie ^{[1
]}

Wandinger-Ness, Angela ^{[1
,2
]}

机构：

[1] Univ New Mexico, Sch Med, Dept Pathol, Albuquerque, NM 87131 USA

[2] Univ New Mexico, Sch Med, Ctr Canc, Albuquerque, NM 87131 USA

[3] Univ New Mexico, Sch Med, Ctr Infect Dis & Immun, Albuquerque, NM 87131 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2013年 / 442卷 / 02期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

Rho; Rac; Cdc42; Rab GTPases; Hantavirus; lntegrins; DECAY-ACCELERATING FACTOR; BCL-2 FAMILY PROTEINS; RHO-GTPASES; NUCLEOTIDE-BINDING; GROWTH-FACTOR; CELL; ACTIVATION; KINASE; RAS; PATHWAY;

D O I：

10.1016/j.ab.2013.07.039

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a rapid assay for measuring the cellular activity of small guanine triphosphatases (GTPases) in response to a specific stimulus. Effector-functionalized beads are used to quantify in parallel multiple GTP-bound GTPases in the same cell lysate by flow cytometry. In a biologically relevant example, five different Ras family GTPases are shown for the first time to be involved in a concerted signaling cascade downstream of receptor ligation by Sin Nombre hantavirus. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：149 / 157

页数：9

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