Recognition of Chromosomal DNA inside Cells by Locked Nucleic Acids

被引：24

作者：

Beane, Randall ^{[1
,2
]}

Gabillet, Sylvie ^{[3
]}

Montaillier, Christophe ^{[3
]}

Arar, Khalil ^{[3
]}

Corey, David R. ^{[1
,2
]}

机构：

[1] Univ Texas SW Med Ctr Dallas, Dept Pharmacol, Dallas, TX 75390 USA

[2] Univ Texas SW Med Ctr Dallas, Dept Biochem, Dallas, TX 75390 USA

[3] SIGMA Aldrich Genopole Campus 1 5, F-91030 Evry, France

来源：

BIOCHEMISTRY | 2008年 / 47卷 / 50期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1021/bi801930p

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sequence-selective recognition of DNA inside cells by oligonucleotides would provide valuable insights into cellular processes and new leads for therapeutics. Recent work, however, has shown that noncoding RNA transcripts overlap chromosomal DNA. These RNAs provide alternate targets for oligonucleotides designed to bind promoter DNA, potentially overturning previous assumptions about mechanism. Here, we show that antigene locked nucleic acids (agLNAs) reduce RNA levels of targeted genes, block RNA polymerase and transcription factor association at gene promoters, and bind to chromosomal DNA. These data suggest that the mechanism of LNAs involves recognition of chromosomal DNA and that LNAs are bona fide antigene molecules.

引用

页码：13147 / 13149

页数：3

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