Immobilization of whole cell penicillin G acylase in open pore gelatin matrix

被引：33
|
作者：
Norouzian, D [1 ]
Javadpour, S
Moazami, N
Akbarzadeh, A
机构：
[1] Pasteur Inst Iran, Pilot Biotechnol Dept, Tehran 13164, IR, Iran
[2] IROST, Biotechnol Res Ctr, Tehran, IR, Iran
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 2002年 / 30卷 / 01期
关键词：
whole cell penicillin G acylase; immobilization;
D O I：
10.1016/S0141-0229(01)00445-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Permeabilization of the cells of E. coli with N-cetyl-N, N, N-trimethylammoniumbromide (CTAB) showed 40% increase in penicillin G acylase activity. The treated cells were immobilized in porous beads of gelatin cross-linked with glutaraldehyde. Upon entrapment the catalytic properties of enzyme were changed as compared to non-immobilized form. Hydrolysis of penicillin G by immobilized system showed 60% conversion of substrate to 6-aminopenicillanic acid in batch system, whereas in continuous system, the conversion rate was 85%. The immobilized form of whole cell penicillin acylase showed low K-m toward penicillin G as a substrate. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码：26 / 29
页数：4
相关论文
共 50 条
  • [1] Immobilization of permeabilized whole cell penicillin G acylase from Alcaligenes faecalis using pore matrix crosslinked with glutaraldehyde
    Cheng, Shiwei
    Wei, Dongzhi
    Song, Qingxun
    Zhao, Xiangguo
    BIOTECHNOLOGY LETTERS, 2006, 28 (14) : 1129 - 1133
  • [2] Immobilization of permeabilized whole cell penicillin G acylase from Alcaligenes faecalis using pore matrix crosslinked with glutaraldehyde
    Shiwei Cheng
    Dongzhi Wei
    Qingxun Song
    Xiangguo Zhao
    Biotechnology Letters, 2006, 28 : 1129 - 1133
  • [3] Immobilization of whole-cell penicillin g acylase by entrapping within polymethacrylamide beads
    Liang-Tzung Hsiau
    Wen-Chien Lee
    Feng-Sheng Wang
    Applied Biochemistry and Biotechnology, 1997, 62 : 303 - 315
  • [4] Immobilization of whole-cell penicillin G acylase by entrapping within polymethacrylamide beads
    Hsiau, LT
    Lee, WC
    Wang, FS
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1997, 62 (2-3) : 303 - 315
  • [5] Paramagnetic epoxy-functionalized mesostructured cellular foams with an open pore system for immobilization of penicillin G acylase
    Yang, Ling
    Gao, Zhenyuan
    Guo, Yanglong
    Zhan, Wangcheng
    Guo, Yun
    Wang, Yunsong
    Lu, Guanzhong
    MICROPOROUS AND MESOPOROUS MATERIALS, 2014, 190 : 17 - 25
  • [6] Design of large-pore mesoporous materials for immobilization of penicillin G acylase biocatalyst
    Chong, ASM
    Zhao, XS
    CATALYSIS TODAY, 2004, 93-5 : 293 - 299
  • [7] THE IMMOBILIZATION OF PENICILLIN-G ACYLASE ON CHITOSAN
    BRAUN, J
    LECHANU, P
    LEGOFFIC, F
    BIOTECHNOLOGY AND BIOENGINEERING, 1989, 33 (02) : 242 - 246
  • [8] Immobilization of penicillin G acylase by entrapment in a sol-gel matrix with magnetic properties
    Bernardino, Susana M. S. A.
    Gallegos, Juan F. M.
    Santhagunam, Aruna
    de Barros, Dragana P. C.
    Fernandes, Pedro
    Fonseca, Luis P.
    JOURNAL OF BIOTECHNOLOGY, 2007, 131 (02) : S97 - S97
  • [9] Immobilization of modified penicillin G acylase on Sepabeads carriers
    Milena Žuža
    Nenad Milosavić
    Zorica Knežević-Jugović
    Chemical Papers, 2009, 63
  • [10] Studies on Immobilization of Penicillin G Acylase to Epoxy Resin
    Fan Chao
    Li Ji-lie
    Zhu Xiao-yuan
    Li Nan
    Wang Wei
    Guo Lu
    Luo Qian
    RENEWABLE AND SUSTAINABLE ENERGY II, PTS 1-4, 2012, 512-515 : 1699 - 1711
12345