Molecular detection of hepatitis E virus in pig livers destined for human consumption in the state of Nuevo Leon, Mexico

被引：7

作者：

Antonio Cantu-Martinez, Marco ^{[1
]}

Xavier Roig-Sagues, Artur ^{[2
]}

Cedillo-Rosales, Sibilina ^{[1
]}

Elisa Zamora-Avila, Diana ^{[1
]}

Avalos-Ramirez, Ramiro ^{[1
]}

机构：

[1] Univ Autonoma Nuevo Leon, Fac Med Vet & Zootecnia, Dept Virol, Col Ex Hacienda El Canad 66050, Nuevo Leon, Mexico

[2] Univ Autonoma Barcelona, Fac Vet, Dept Ciencia Anim & Aliments, E-08193 Barcelona, Spain

来源：

SALUD PUBLICA DE MEXICO | 2013年 / 55卷 / 02期

关键词：

hepatitis E; HEV; pork livers; Mexico; SWINE; JAPAN;

D O I：

10.1590/S0036-36342013000200011

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Objective. Molecular detection of HEY in pig livers destined for human consumption in Nuevo Leon, Mexico. Materials and methods. 87 livers were collected from pigs slaughtered in TIF and 40 livers from butchers. A 212 pb fragment of HEY ORF2 gene was amplified by semi-nested RT-PCR. Results. 19.54% (17) of tif's and 22.5% (9) of buthcer's livers were positive for HEY. Sequencing of the amplified products showed a 94%-95% homology with the sequences reported for genotype 3. Conclusions. Our results indicate that HEY is circulating in swine herds in the state, constituting a probable source of contamination of pig meat products.

引用

页码：193 / 195

页数：3

共 14 条

[1]

[Anonymous], 2007, SIST UN INF VIG EP D

[2] Hepatitis E virus antibody prevalence among persons who work with swine [J].

Drobeniuc, J ;

Favorov, MO ;

Shapiro, CN ;

Bell, BP ;

Mast, EE ;

Dadu, A ;

Culver, D ;

Iarovoi, P ;

Robertson, BH ;

Margolis, HS .

JOURNAL OF INFECTIOUS DISEASES, 2001, 184 (12) :1594-1597

[3] Comparative pathogenesis of infection of pigs with hepatitis E viruses recovered from a pig and a human [J].

Halbur, PG ;

Kasorndorkbua, C ;

Gilbert, C ;

Guenette, D ;

Potters, MB ;

Purcell, RH ;

Emerson, SU ;

Toth, TE ;

Meng, XJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (03) :918-923

[4] Identity of a novel swine hepatitis E virus in Taiwan forming a monophyletic group with Taiwan isolates of human hepatitis E virus [J].

Hsieh, SY ;

Meng, XJ ;

Wu, YH ;

Liu, ST ;

Tam, AW ;

Lin, DY ;

Liaw, YF .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (12) :3828-3834

[5] Detection by reverse transcription-PCR and genetic characterization of field isolates of swine hepatitis E virus from pigs in different geographic regions of the united states [J].

Huang, FF ;

Haqshenas, G ;

Guenette, DK ;

Halbur, PG ;

Schommer, SK ;

Pierson, FW ;

Toth, TE ;

Meng, XJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (04) :1326-1332

[6] Acute hepatitis caused by a novel strain of hepatitis E virus most closely related to United States strains [J].

Kabrane-Lazizi, Y ;

Zhang, MD ;

Purcell, RH ;

Miller, KD ;

Davey, RT ;

Emerson, SU .

JOURNAL OF GENERAL VIROLOGY, 2001, 82 :1687-1693

[7] Phylogenetic analysis of global hepatitis E virus sequences: genetic diversity, subtypes and zoonosis [J].

Lu, L ;

Li, CH ;

Hagedorn, CH .

REVIEWS IN MEDICAL VIROLOGY, 2006, 16 (01) :5-36

[8] Association of hepatitis E virus (HEV) and postweaning multisystemic wasting syndrome (PMWS) with lesions of hepatitis in pigs [J].

Martin, M. ;

Segales, J. ;

Huang, F. F. ;

Guenette, D. K. ;

Mateu, E. ;

de Deus, N. ;

Meng, X. J. .

VETERINARY MICROBIOLOGY, 2007, 122 (1-2) :16-24

[9] Prevalence of antibodies to hepatitis E virus in veterinarians working with swine and in normal blood donors in the United States and other countries [J].

Meng, XJ ;

Wiseman, B ;

Elvinger, F ;

Guenette, DK ;

Toth, TE ;

Engle, RE ;

Emerson, SU ;

Purcell, RH .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (01) :117-122

[10] A novel virus in swine is closely related to the human hepatitis E virus [J].

Meng, XJ ;

Purcell, RH ;

Halbur, PG ;

Lehman, JR ;

Webb, DM ;

Tsareva, TS ;

Haynes, JS ;

Thacker, BJ ;

Emerson, SU .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (18) :9860-9865

← 1 2 →