Marinomonas mangrovi sp nov., isolated from mangrove sediment

A Gram-stain-negative, Na+ -requiring bacterial strain, designated B20-1(T), was isolated from soil of the root system of mangrove forest. Cells were curved rods and motile by means of a polar flagellum. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain B20-1T belonged to the genus Marinomonas, sharing highest sequence similarities with Marinomonas rhizomae IVIA-Po-145(T) (97.6%), Marinomonas dokdonensis DSW10-10(T) (97.0 %) and Marinomonas foliarum IVIA-Po-155(T) (96.9%). The predominant cellular fatty acids of strain B20-1(T) were C-10:0 3-OH, C-18:1 omega 7c, summed feature 3 (C-(16:1)omega 7c and/or iso-C-15:0 2-OH) and C-16:0. Phosphatidylethanolamine and phosphatidylglycerol were identified as the predominant phospholipids. The predominant ubiquinone was Q-8. The genomic DNA G+C content of strain B20-1(T) was 46.6 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA DNA relatedness, a novel species, Marinomonas mangrovi sp. nov., is proposed with B20-1(T) (=DSM 28136(T)=LMG 28077(T)) as the type strain.