A precision cryostat design for manual and semi-automated cryo-plunge instruments

被引：30

作者：

Russo, Christopher J. ^{[1
]}

Scotcher, Steve ^{[1
]}

Kyte, Martin ^{[1
]}

机构：

[1] MRC Lab Mol Biol, Francis Crick Ave, Cambridge CB2 0QH, England

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 2016年 / 87卷 / 11期

基金：

英国医学研究理事会;

关键词：

ELECTRON CRYOMICROSCOPY; MICROSCOPY;

D O I：

10.1063/1.4967864

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Here we describe a bench-top cryostat system to control the temperature of liquid ethane in a cryo-plunge apparatus designed for biological specimen preparation for electron cryomicroscopy. It comprises a foam insulated Dewar containing a copper cryostat cup, whose temperature is controlled via an active feedback system to within 0.1 K. The device can easily be incorporated into existing manual and semi-automatic cryo-plunge instruments that are not equipped with cryogenic temperature control. Over the course of normal use, we find that using a cryostat is convenient, fast, and does not require special mixtures of cryogens like ethane/propane. This simple cryostat improves the reliability and reproducibility of biological specimen preparation for electron cryomicroscopy. (C) 2016 Author(s).

引用

页数：5

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