Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders

被引：49
作者：
Crespilho, A. M. [2 ]
Sa Filho, M. F. [3 ]
Dell'Aqua, J. A., Jr. [1 ]
Nichi, M. [3 ]
Monteiro, G. A. [1 ]
Avanzi, B. R. [1 ]
Martins, A. [4 ]
Papa, F. O. [1 ]
机构：
[1] Sao Paulo State Univ, Dept Anim Reprod & Vet Radiol, Botucatu, SP, Brazil
[2] Univ Santo Amaro, UNISA, Sch Vet Med, Sao Paulo, Brazil
[3] Univ Sao Paulo, Dept Anim Reprod, Sao Paulo, Brazil
[4] Sao Paulo State Univ, Dept Clin Surg & Anim Reprod, Aracatuba, SP, Brazil
来源：
LIVESTOCK SCIENCE | 2012年 / 149卷 / 1-2期
基金：
巴西圣保罗研究基金会;
关键词：
Bull semen; Semen extender; Egg yolk; Soy lecithin; Cryopreservation; Artificial insemination; SPERM CRYOPRESERVATION; PARAMETERS; QUALITY;
D O I：
10.1016/j.livsci.2012.05.011
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
One of the main factors known to influence quality and fertility of bovine cryopreserved semen is the extender used. In this matter, a great worldwide interest has been directed to the development of chemically defined media, free of animal origin products. The objective of the present study was to compare the efficacy of three bovine semen extenders: Iris-fructose (IRIS, control with 20% egg yolk), Botu-Bov (R) (BB; 20% egg yolk), and Botu-Bov (R)-soy lecithin (BB-L; 1% soy lecithin). Towards this aim, post-thaw computer assisted sperm analysis (CASA), sperm membrane and acrosome integrity were evaluated (Experiment 1). Additionally, cryopreserved samples were used in a fixed time artificial insemination program aiming to evaluate in vivo fertility (pregnancy per insemination-P/AI; Experiment 2). Despite the higher straightness and linearity found for samples cryopreserved in BB and BB-L when compared to those cryopreserved in IRIS, egg yolk based extenders provided higher total and progressive motilities, percentage of rapid sperms and intact membrane cells (P < 0.05). Furthermore, P/IA was higher in samples cryopreserved in egg yolk based extenders when compared to soy lecithin [TRIS=59.26(a) (64/108), BB=62.37(a) (58/93), and BB-L=36.45(b) (35/96)]. Although soy lecithin represents an alternative for the development of chemically defined extenders with decreased risk of biological contamination, egg yolk based extenders are more efficient on the preservation of bovine post-thaw sperm viability and fertility. (C) 2012 Elsevier B.V. All rights reserved.
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页码：1 / 6
页数：6
相关论文
共 28 条
[1]   In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen [J].
Aires, VA ;
Hinsch, KD ;
Mueller-Schloesser, F ;
Bogner, K ;
Mueller-Schloesser, S ;
Hinsch, E .
THERIOGENOLOGY, 2003, 60 (02) :269-279
[2]   Cryopreservation of buffalo (Bubalus bubalis) semen in Bioxcell® extender [J].
Akhter, S. ;
Ansari, M. S. ;
Rakha, B. A. ;
Andrabi, S. M. H. ;
Iqbal, S. ;
Ullah, N. .
THERIOGENOLOGY, 2010, 74 (06) :951-955
[3]   Modifications of bull spermatozoa induced by three extenders: Biociphos, low density lipoprotein and Triladyl, before, during and after freezing and thawing [J].
Amirat, L ;
Anton, M ;
Tainturier, D ;
Chatagnon, G ;
Battut, I ;
Courtens, JL .
REPRODUCTION, 2005, 129 (04) :535-543
[4]   Validation of body condition score as a predictor of subcutaneous fat in Nelore (Bos indicus) cows [J].
Ayres, Henderson ;
Ferreira, Roberta Machado ;
de Souza Torres-Junior, Jose Ribamar ;
Borges Demetrio, Clarice Garcia ;
de Lima, Cesar Goncalves ;
Baruselli, Pietro Sampaio .
LIVESTOCK SCIENCE, 2009, 123 (2-3) :175-179
[5]   Comparison of bacteriological qualities of various egg yolk sources and the in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or lecithin based diluents [J].
Bousseau, S ;
Brillard, JP ;
Marquant-Le Guienne, B ;
Guérin, B ;
Camus, A ;
Lechat, M .
THERIOGENOLOGY, 1998, 50 (05) :699-706
[6]   Effects that bovine sperm cryopreservation using two different extenders has on sperm membranes and chromatin [J].
Celeghini, Eneiva Carla Carvalho ;
de Arruda, Rubens Paes ;
de Andrade, Andre Furugen Cesar ;
Nascimento, Juliana ;
Raphael, Claudia Fernandes ;
Rodrigues, Paulo Henrique Mazza .
ANIMAL REPRODUCTION SCIENCE, 2008, 104 (2-4) :119-131
[7]   Improvement of parameters of freezing medium and freezing protocol for bull sperm using two osmotic supports [J].
Chaveiro, A. ;
Machado, L. ;
Frijters, A. ;
Engel, B. ;
Woelders, H. .
THERIOGENOLOGY, 2006, 65 (09) :1875-1890
[8]  
Crespilho A. M., 2006, Ars Veterinaria, V22, P229
[9]   Fertility evaluation in Egyptian buffalo bulls using zona pellucida binding and in vitro fertilization assays [J].
Dayem, A. M. H. Abdel ;
Mahmoud, Karima Gh. M. ;
Nawito, M. F. ;
Ayoub, M. M. ;
Scholkamy, T. H. .
LIVESTOCK SCIENCE, 2009, 122 (2-3) :193-198
[10]   Ways to improve the biosecurity of bovine semen [J].
de Ruigh, L. ;
Bosch, J. C. ;
Brus, M. C. ;
Landman, B. ;
Merton, J. S. .
REPRODUCTION IN DOMESTIC ANIMALS, 2006, 41 (04) :268-274
123