Use of IP-10 detection in dried plasma spots for latent tuberculosis infection diagnosis in contacts via mail

被引：4

作者：

Villar-Hernandez, R. ^{[1
,2
,3
]}

Latorre, I ^{[1
,2
,3
]}

De Souza-Galvao, M. L. ^{[4
]}

Jimenez, M. A. ^{[4
]}

Ruiz-Manzano, J. ^{[2
,3
,5
]}

Pilarte, J. ^{[4
]}

Garcia-Garcia, E. ^{[1
,2
,3
]}

Muriel-Moreno, B. ^{[1
,2
,3
]}

Cantos, A. ^{[4
]}

Altet, N. ^{[4
,6
,7
]}

Millet, J. P. ^{[6
,7
]}

Gonzalez-Diaz, Y. ^{[6
]}

Molina-Pinargote, I ^{[6
]}

Prat, C. ^{[1
,2
,3
]}

Ruhwald, M. ^{[8
]}

Dominguez, J. ^{[1
,2
,3
]}

机构：

[1] Hosp Badalona Germans Trias & Pujol, Inst Invest Germans Trias & Pujol, Serv Microbiol, Carretera Canyet, Barcelona 08916, Spain

[2] Inst Salud Carlos III, CIBERES, CIBER Enfermedades Resp, Carretera Canyet, Barcelona 08916, Spain

[3] Univ Autonoma Barcelona, Carretera Canyet, Barcelona 08916, Spain

[4] Hosp Univ Vall dHebron, Unitat TB Drassanes, Av Drassanes 17, Barcelona 08001, Barcelona, Spain

[5] Hosp Badalona Germans Trias & Pujol, Serv Pneumol, Carretera Canyet, Barcelona 08916, Spain

[6] Unidad Clin Tratamiento Directamente Observ Serv, Carrer Garcia Marino 4, Barcelona 08022, Spain

[7] Inst Salud Carlos III, CIBEREESP, CIBER Epidemiol & Salud PUbl, Carretera Canyet, Barcelona 08916, Spain

[8] Statens Serum Inst, Dept Infect Dis Immunol, Artillerivej 5, DK-2300 Copenhagen, Denmark

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

INDUCIBLE PROTEIN 10; IFN-GAMMA; MYCOBACTERIUM-TUBERCULOSIS; RELEASE ASSAYS; SKIN-TEST; BIOMARKER; MARKER;

D O I：

10.1038/s41598-019-40778-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of this study was to test the use of IP-10 detection in dried plasma from contact studies individuals (contacts of smear positive patients), by comparing it with IP-10 and IFN-gamma detection in direct plasma, to establish IP-10 detection in DPS as a useful assay for LTBI diagnosis. Whole blood samples were collected from 80 subjects: 12 with active tuberculosis (TB), and 68 from contact studies. The amount of IFN-gamma produced by sensitized T cells was determined in direct plasma by QuantiFERON Gold In-Tube test. IP-10 levels were determined in direct and dried plasma by an in-house ELISA. For dried plasma IP-10 determination, two 25 mu l plasma drops were dried in Whatman903 filter paper and sent by mail to the laboratory. Regarding TB patients, 100.0%, 91.7% and 75.0% were positive for IFN-gamma detection and IP-10 detection in direct and dried plasma, respectively. In contacts, 69.1%, 60.3% and 48.5% had positive results after IFN-gamma and IP-10 in direct and dried plasma, respectively. The agreement among in vitro tests was substantial and IP-10 levels in direct and dried plasma were strongly correlated (r = 0.897). In conclusion, IP-10 detection in dried plasma is a simple and safe method that would help improve LTBI management.

引用

页数：11

共 32 条

[31] Diagnosis of latent tuberculosis infection among pediatric household contacts of Iranian tuberculosis cases using tuberculin skin test, IFN- γ release assay and IFN-γ-induced protein-10
Roxana Mansour Ghanaie
Abdollah Karimi
Leila Azimi
Seddon James
Mahshid Nasehi
Abolfazl Panahi Mishkar
Mahnaz Sheikhi
Fatemeh Fallah
Sedigheh Rafiei Tabatabaei
Seyedeh Mahsan Hoseini-Alfatemi
BMC Pediatrics, 21
[32] Diagnosis of latent tuberculosis infection among pediatric household contacts of Iranian tuberculosis cases using tuberculin skin test, IFN- γ release assay and IFN-γ-induced protein-10
Ghanaie, Roxana Mansour
Karimi, Abdollah
Azimi, Leila
James, Seddon
Nasehi, Mahshid
Mishkar, Abolfazl Panahi
Sheikhi, Mahnaz
Fallah, Fatemeh
Tabatabaei, Sedigheh Rafiei
Hoseini-Alfatemi, Seyedeh Mahsan
BMC PEDIATRICS, 2021, 21 (01)

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