CD and MCD studies of the non-heme ferrous active site in (4-hydroxyphenyl)pyruvate dioxygenase:: Correlation between oxygen activation in the extradiol and α-KG-dependent dioxygenases

被引：51

作者：

Neidig, ML

Kavana, M

Moran, GR

Solomon, EI ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[2] Univ Wisconsin, Dept Chem & Biochem, Milwaukee, WI 53211 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 14期

关键词：

D O I：

10.1021/ja0316521

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(4-Hydroxyphenyl)pyruvate dioxygenase (HPPD) is an unusual α-keto acid-dependent non-heme iron dioxygenase as it incorporates both atoms of dioxygen into a single substrate, paralleling the extradiol dioxygenases. CD/MCD studies of the catalytically active ferrous site and its interaction with substrate reveal a geometic and electronic structure and mechanistic approach to oxygen activation which bridges those of the α-KG-dependent and the extradiol dioxygenases. Copyright © 2004 American Chemical Society.

引用

收藏

页码：4486 / 4487

页数：2

相关论文

共 10 条

[1]

BRADLEY FC, 1986, J BIOL CHEM, V261, P1693

[2] Spectroscopic and electronic structure studies of 2,3-dihydroxybiphenyl 1,2-dioxygenase:: O2 reactivity of the non-heme ferrous site in extradiol dioxygenases [J].

Davis, MI ;

Wasinger, EC ;

Decker, A ;

Pau, MYM ;

Vaillancourt, FH ;

Bolin, JT ;

Eltis, LD ;

Hedman, B ;

Hodgson, KO ;

Solomon, EI .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (37) :11214-11227

[3] (4-hydroxyphenyl)pyruvate dioxygenase from Streptomyces avermitilis:: The basis for ordered substrate addition [J].

Johnson-Winters, K ;

Purpero, VM ;

Kavana, M ;

Nelson, T ;

Moran, GR .

BIOCHEMISTRY, 2003, 42 (07) :2072-2080

[4]

NEIDIG ML, UNPUB

[5] Circular dichroism and magnetic circular dichroism spectroscopic studies of the non-heme ferrous active site in clavaminate synthase and its interaction with α-ketoglutarate cosubstrate [J].

Pavel, EG ;

Zhou, J ;

Busby, RW ;

Gunsior, M ;

Townsend, CA ;

Solomon, EI .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (04) :743-753

[6] Magnetic circular dichroism spectroscopic studies of mononuclear non-heme ferrous model complexes. Correlation of excited- and ground-state electronic structure with geometry [J].

Pavel, EG ;

Kitajima, N ;

Solomon, EI .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (16) :3949-3962

[7] Crystal structure of Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase:: an enzyme involved in the tyrosine degradation pathway [J].

Serre, L ;

Sailland, A ;

Sy, D ;

Boudec, P ;

Rolland, A ;

Pebay-Peyroula, E ;

Cohen-Addad, C .

STRUCTURE, 1999, 7 (08) :977-988

[8] Geometric and electronic structure/function correlations in non-heme iron enzymes [J].

Solomon, EI ;

Brunold, TC ;

Davis, MI ;

Kemsley, JN ;

Lee, SK ;

Lehnert, N ;

Neese, F ;

Skulan, AJ ;

Yang, YS ;

Zhou, J .

CHEMICAL REVIEWS, 2000, 100 (01) :235-349

[9] MAGNETIC CIRCULAR-DICHROISM SPECTROSCOPY AS A PROBE OF THE GEOMETRIC AND ELECTRONIC-STRUCTURE OF NONHEME FERROUS ENZYMES [J].

SOLOMON, EI ;

PAVEL, EG ;

LOEB, KE ;

CAMPOCHIARO, C .

COORDINATION CHEMISTRY REVIEWS, 1995, 144 :369-460

[10] Spectroscopic studies of substrate interactions with clavaminate synthase 2, a multifunctional α-KG-dependent non-heme iron enzyme:: Correlation with mechanisms and reactivities [J].

Zhou, J ;

Kelly, WL ;

Bachmann, BO ;

Gunsior, M ;

Townsend, CA ;

Solomon, EI .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (30) :7388-7398