Optical Properties of Buffers and Cell Culture Media for Optofluidic and Sensing Applications

被引：49

作者：

Van Thuy Hoang ^{[1
,2
]}

Stepniewski, Grzegorz ^{[1
,2
]}

Czarnecka, Karolina H. ^{[3
,4
]}

Kasztelanic, Rafal ^{[1
,2
]}

Long, Van Cao ^{[5
]}

Khoa Dinh Xuan ^{[6
]}

Shao, Liyang ^{[7
]}

Smietana, Mateusz ^{[3
,7
]}

Buczynski, Ryszard ^{[1
,2
]}

机构：

[1] Univ Warsaw, Fac Phys, Pasteura 7, PL-02093 Warsaw, Poland

[2] Inst Elect Mat Technol, Wolczynska 133, PL-01919 Warsaw, Poland

[3] Warsaw Univ Technol, Inst Microelect & Optoelect, Koszykowa 75, PL-00662 Warsaw, Poland

[4] Med Univ Lodz, Dept Biomed & Genet, Pomorska 251, PL-92213 Lodz, Poland

[5] Univ Zielona Gora, Inst Phys, Prof Szafrana 4a, PL-65516 Zielona Gora, Poland

[6] Vinh Univ, Dept Phys, Le Duan 182, Vinh 420000, Nghe An, Vietnam

[7] Southern Univ Sci & Technol, Dept Elect & Elect Engn, Shenzhen 518055, Peoples R China

来源：

APPLIED SCIENCES-BASEL | 2019年 / 9卷 / 06期

基金：

中国国家自然科学基金;

关键词：

optical properties; cell culture media; optofluidics; optical sensing; label-free biosensing; NANOSENSORS; LASER;

D O I：

10.3390/app9061145

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Interactions between light and various cells in cultures, such as bacteria or mammalian cells, are widely applied for optical sensors and optofluidic systems. These microorganisms need to be kept in proper aqueous media, referred to as buffers or cell culture media, that are required, respectively, for stable storage or delivering biochemical nutrients for their growth. When experiments or numerical analyses on optical devices are performed, the properties of these media are usually considered to be similar to those of pure water, with negligible influence of biochemical compounds on the medium's optical properties. In this work, we investigated the transmission, material dispersion, and scattering properties of selected and widely used buffers and cell culture media. We show that the optical properties of these media may significantly vary from those of water. Well-defined properties of buffers and cell culture media are essential for proper design of various optical sensing or future optofluidic systems dealing with biological structures.

引用

页数：11

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