Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

被引：107

作者：

Zhu, Min ^{[1
]}

Heydarkhan-Hagvall, Sepideh ^{[2
]}

Hedrick, Marc ^{[1
]}

Benhaim, Prosper ^{[3
,4
]}

Zuk, Patricia ^{[3
,5
]}

机构：

[1] Cytori Therapeut Inc, San Diego, CA USA

[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Surg, Div Cardiac Surg, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, David Geffen Sch Med, Dept Surg, Div Plast & Reconstruct Surg, Los Angeles, CA 90095 USA

[4] Univ Calif Los Angeles, David Geffen Sch Med, Dept Orthoped Surg, Los Angeles, CA 90095 USA

[5] Univ Calif Los Angeles, David Geffen Sch Med, Regenerat Bioengn & Repair Lab, Los Angeles, CA 90095 USA

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2013年 / 79期

基金：

美国国家卫生研究院;

关键词：

Cellular Biology; Issue; 79; Adipose Tissue; Stem Cells; Humans; Cell Biology; biology (general); enzymatic digestion; collagenase; cell isolation; Stromal Vascular Fraction (SVF); Adipose-derived Stem Cells; ASCs; lipoaspirate; liposuction; BONE-MARROW; TISSUE; YIELD; GROWTH;

D O I：

10.3791/50585

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In 2001, researchers at the University of California, Los Angeles, described the isolation of a new population of adult stem cells from liposuctioned adipose tissue that they initially termed Processed Lipoaspirate Cells or PLA cells. Since then, these stem cells have been renamed as Adipose-derived Stem Cells or ASCs and have gone on to become one of the most popular adult stem cells populations in the fields of stem cell research and regenerative medicine. Thousands of articles now describe the use of ASCs in a variety of regenerative animal models, including bone regeneration, peripheral nerve repair and cardiovascular engineering. Recent articles have begun to describe the myriad of uses for ASCs in the clinic. The protocol shown in this article outlines the basic procedure for manually and enzymatically isolating ASCs from large amounts of lipoaspirates obtained from cosmetic procedures. This protocol can easily be scaled up or down to accommodate the volume of lipoaspirate and can be adapted to isolate ASCs from fat tissue obtained through abdominoplasties and other similar procedures.

引用

页数：10

共 36 条

[11] Human adipose stem cells: A potential cell source for cardiovascular tissue engineering [J].