Pneumocystis carinii telomere repeats are composed of TTAGGG and the subtelomeric sequence contains a gene encoding the major surface glycoprotein

被引：43

作者：

Underwood, AP

Louis, EJ

Borts, RH

Stringer, JR

Wakefield, AE

机构：

[1] JOHN RADCLIFFE HOSP,INST MOLEC MED,DEPT PAEDIAT,MOLEC INFECT DIS GRP,OXFORD OX3 9DU,ENGLAND

[2] JOHN RADCLIFFE HOSP,INST MOLEC MED,YEAST GENET GRP,OXFORD OX3 9DU,ENGLAND

[3] UNIV CINCINNATI,COLL MED,DEPT MOLEC GENET BIOCHEM & MICROBIOL,CINCINNATI,OH 45267

来源：

MOLECULAR MICROBIOLOGY | 1996年 / 19卷 / 02期

基金：

英国惠康基金;

关键词：

D O I：

10.1046/j.1365-2958.1996.374904.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned a telomere and adjacent sequences from rat-derived Pneumocystis carinii using the ability of foreign telomeres to complement a yeast artificial chromosome (YAC) deficient by one telomere in Saccharomyces cerevisiae. Characterization of the cloned DNA in the recombinant YAC demonstrated that it was a chimera of two P. carinii sequences, namely a 13.5 kb fragment of mitochondrial DNA and an 8.3 kb distal portion consisting of subtelomeric DNA. The P. carinii telomere repeat was demonstrated to be TTAGGG, the most common telomere repeat found in organisms from the animal and fungal kingdoms. Karyotype analysis confirmed that this sequence was present on all the P. carinii chromosomes. Sequence adjacent to the telomere repeats was shown by Bal3l exonuclease digestion to be located at the chromosome ends. Analysis of the subtelomeric fragment revealed homology to the gene encoding the major surface glycoprotein of P. carinii.

引用

页码：273 / 281

页数：9