Determination of morphine by capillary zone electrophoresis immunoassay combined with laser-induced fluorescence detection

被引：27

作者：

Mi, JQ ^{[1
]}

Zhang, XX ^{[1
]}

Chang, WB ^{[1
]}

机构：

[1] Peking Univ, Coll Chem & Mol Engn, Dept Biol Chem, Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China

来源：

JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY | 2004年 / 25卷 / 01期

关键词：

D O I：

10.1081/IAS-120027226

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A competitive immunoassay for detecting morphine in bio-samples was established by capillary zone electrophoresis combined with laser-induced fluorescence detection (CZE-LIF); The antigen of morphine was labeled with isothiocyano-fluorescein (FITC) and then incubated with morphine monoclonal antibody and samples. The linear range was 50-1000 ng/mL, which was suitable for clinical and forensic, applications. The detection limit can reach 40 ng/mL, based on S/N = 2. The. recoveries of morphine from serum were satisfactory.

引用

页码：57 / 70

页数：14

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