Antifungal activity of transparent nanocomposite thin films of pullulan and silver against Aspergillus niger

被引:93
作者
Pinto, Ricardo J. B. [1 ,2 ]
Almeida, Adelaide [3 ,4 ]
Fernandes, Susana C. M. [1 ,2 ]
Freire, Carmen S. R. [1 ,2 ]
Silvestre, Armando J. D. [1 ,2 ]
Pascoal Neto, Carlos [1 ,2 ]
Trindade, Tito [1 ,2 ]
机构
[1] Univ Aveiro, CICECO, P-3810193 Aveiro, Portugal
[2] Univ Aveiro, Dept Chem, P-3810193 Aveiro, Portugal
[3] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal
[4] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal
关键词
Nanocomposites; Pullulan; Silver nanoparticles; Antifungal activity; Aspergillus niger; ANTIBACTERIAL ACTIVITY; NANOPARTICLES; INFECTIONS; POLYMERS; AGENTS;
D O I
10.1016/j.colsurfb.2012.09.045
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Silver has been mainly investigated as an antibacterial agent and less as a fungicide in which concerns antimicrobial properties. In this research, the antifungal activity of composite films of pullulan and Ag nanoparticles (NP) against Aspergillus niger was evaluated using standard protocols. These new materials were prepared as transparent cast films (66-74 mu m thickness) from Ag hydrosols containing the polysaccharide. Fungal growth inhibition was observed in the presence of such silver nanocomposite films. Moreover, disruption of the spores cells of A. niger was probed for the first time by means of scanning electron microscopy (SEM). This effect occurred in the presence of the nanocomposites due to Ag NP dispersed as fillers in pullulan. This polysaccharide was used here as a biocompatible matrix, hence making these nanocomposites beneficial for the development of antifungal packaging materials. (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:143 / 148
页数:6
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