Simultaneous Detection of Seven Enteric Viruses Associated with Acute Gastroenteritis by a Multiplexed Luminex-Based Assay

被引：53

作者：

Liu, Yan ^{[1
]}

Xu, Zi-qian ^{[1
]}

Zhang, Qing ^{[1
]}

Jin, Miao ^{[1
]}

Yu, Jie-mei ^{[1
]}

Li, Jin-song ^{[1
]}

Liu, Na ^{[1
]}

Cui, Shu-xian ^{[1
]}

Kong, Xiang-yu ^{[1
]}

Wang, Hong ^{[1
]}

Li, Hui-ying ^{[1
]}

Cheng, Wei-xia ^{[1
]}

Ma, Xue-jun ^{[1
]}

Duan, Zhao-jun ^{[1
]}

机构：

[1] China CDC, Natl Inst Viral Dis Control & Prevent, State Key Lab Mol Virol & Genet Engn, Beijing, Peoples R China

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 07期

关键词：

HUMAN BOCAVIRUS INFECTION; VIRAL GASTROENTERITIS; MOLECULAR EPIDEMIOLOGY; HUMAN PARECHOVIRUS; CAUSATIVE AGENTS; CHILDREN; NOROVIRUS; IDENTIFICATION; PREVALENCE; DIARRHEA;

D O I：

10.1128/JCM.06790-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Rapid and broad diagnostic methods are needed for the identification of viral agents of gastroenteritis. In this study, we used Luminex xMAP technology to develop a multiplexed assay for the simultaneous identification of major enteric viral pathogens, including rotavirus A (RVA), noroviruses (NoVs) (including genogroups GI and GII), sapoviruses (SaV), human astrovirus (HAstV), enteric adenoviruses (EAds), and human bocavirus 2 (HBoV2). The analytical sensitivity allowed detection of 10(3) (EAds, HBoV2, and RVA) and 10(4) (NoV GI and GII, SaV, and HAstV) copies per reaction mixture. Compared to conventional PCR, the Luminex-based assay yielded greater than 75% sensitivity and 97% specificity for each virus, and the kappa correlation for detection of all viruses ranged from 0.75 to 1.00. In conclusion, this multiplexed Luminex-based assay provides a potentially rapid, high-throughput, and maneuverable diagnostic tool for major viral pathogens associated with gastroenteritis.

引用

页码：2384 / 2389

页数：6

共 33 条

[1] Viral gastroenteritis. An epidemiologic investigation between the period 2001-2006 [J].

Allwinn R. ;

Janz B. ;

Doerr H.W. .

Medizinische Klinik, 2008, 103 (6) :389-395

[2] Development and evaluation of a Luminex multiplex serology assay to detect antibodies to bovine herpes virus 1, parainfluenza 3 virus, bovine viral diarrhoea virus, and bovine respiratory syncytial virus, with comparison to existing ELISA detection methods [J].

Anderson, Steve ;

Wakeley, Phil ;

Wibberley, Guy ;

Webster, Kath ;

Sawyer, Jason .

JOURNAL OF IMMUNOLOGICAL METHODS, 2011, 366 (1-2) :79-88

[3] Human Bocavirus in Children [J].

Arnold, John C. .

PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2010, 29 (06) :557-558

[4]

Bailey M S, 2008, J R Army Med Corps, V154, P156

[5] High Prevalence of Human Parechovirus (HPeV) Genotypes in the Amsterdam Region and Identification of Specific HPeV Variants by Direct Genotyping of Stool Samples [J].

Benschop, K. ;

Thomas, X. ;

Serpenti, C. ;

Molenkamp, R. ;

Wolthers, K. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (12) :3965-3970

[6] Human Bocavirus Infection in Children With Acute Respiratory Tract Infection in India [J].

Bharaj, Preeti ;

Sullender, Wayne M. ;

Kabra, Sushil K. ;

Broor, Shobha .

JOURNAL OF MEDICAL VIROLOGY, 2010, 82 (05) :812-816

[7]

Bio-Rad Laboratories, 2010, BIOPL COUPL PROT

[8] Role of Human Bocavirus infections in outbreaks of gastroenteritis [J].

Campe, Hartmut ;

Hartberger, Christine ;

Sing, Andreas .

JOURNAL OF CLINICAL VIROLOGY, 2008, 43 (03) :340-342

[9]

Chieochansin T, 2008, JPN J INFECT DIS, V61, P479

[10] Use of Luminex xMAP-derived Bio-Plex bead-based suspension array for specific detection of PPV W and characterization of epitopes on the coat protein of the virus [J].

Croft, Heather ;

Malinowski, Tadeusz ;

Krizbai, Laszlo ;

Mikec, Ivan ;

Kajic, Vesna ;

Reed, Christopher ;

Varga, Aniko ;

James, Delano .

JOURNAL OF VIROLOGICAL METHODS, 2008, 153 (02) :203-213

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