Colorimetric detection of lysine using gold nanoparticles aggregation

被引：36

作者：

Zhou, Yanli ^{[1
]}

Yang, Zhichong ^{[1
]}

Xu, Maotian ^{[1
]}

机构：

[1] Shangqiu Normal Univ, Dept Chem & Chem Engn, Henan Key Lab Cultivat Base Nanobiol Analyt Chem, Shangqiu 476000, Peoples R China

来源：

ANALYTICAL METHODS | 2012年 / 4卷 / 09期

基金：

中国国家自然科学基金;

关键词：

AMPEROMETRIC BIOSENSOR; CYSTEINE; SENSOR;

D O I：

10.1039/c2ay25475a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple, reliable and sensitive colorimetric method for the detection of lysine was developed based on the use of citrate-stabilized gold nanoparticles (AuNPs). In acid media (pH = 3.0), AuNPs exhibited a sensitive color change from red to blue after the addition of lysine for 30 min at room temperature, which could directly be seen with the naked eye and monitored by UV-vis absorption spectroscopy. Results showed that the absorbance ratio (A(680)/A(520)) was linear for a lysine concentration in the range of 1 to 70 mu M (r = 0.995), and the limit of detection was 0.8 mu M at a signal-to-noise ratio of 3. This method also demonstrated excellent selectivity for lysine over other amino acids. For the analysis of lysine in food samples, precision and accuracy were checked.

引用

页码：2711 / 2714

页数：4

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