Alginate gel microwell arrays using electrodeposition for three-dimensional cell culture

被引：72

作者：

Ozawa, Fumisato ^{[1
,2
]}

Ino, Kosuke ^{[1
]}

Arai, Toshiharu ^{[1
]}

Ramon-Azcon, Javier ^{[2
]}

Takahashi, Yasufumi ^{[2
]}

Shiku, Hitoshi ^{[1
]}

Matsue, Tomokazu ^{[1
,2
]}

机构：

[1] Tohoku Univ, Grad Sch Environm Studies, Sendai, Miyagi 9808579, Japan

[2] Tohoku Univ, WPI Adv Inst Mat Res, Sendai, Miyagi 9808579, Japan

来源：

LAB ON A CHIP | 2013年 / 13卷 / 15期

基金：

日本学术振兴会;

关键词：

EMBRYOID BODIES; RESPIRATION; SYSTEM;

D O I：

10.1039/c3lc50455g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we developed a novel method for fabricating microwell arrays constructed from alginate gels, and the alginate gel microwells were used for three-dimensional (3D) cell culture. The alginate gel microwells were fabricated on a patterned ITO electrode using alginate gel electrodeposition. Embryonic stem (ES) cells or hepatocellular carcinoma cells (HepG2) were cultured in the alginate gel microwells containing 3T3 cells. During the culture, embryoid bodies (EBs) or HepG2 spheroids were successfully fabricated in the alginate gel microwells. The oxygen consumption of the EBs indicated that they were successfully cultured. Liver-specific gene expressions of the HepG2 spheroids apparently increased by performing 3D co-culture in the microwell arrays with 3T3 cells. These results show that the alginate gel microwells are a useful 3D culture system.

引用

页码：3128 / 3135

页数：8

共 27 条

[1] Effect of cell-cell interactions in preservation of cellular phenotype: cocultivation of hepatocytes and nonparenchymal cells [J].