Determination of microscopic acid-base parameters from NMR-pH titrations

被引：104
作者：
Szakács, Z
Kraszni, M
Noszál, B
机构：
[1] Semmelweis Univ, Dept Pharmaceut Chem, H-1092 Budapest, Hungary
[2] Eotvos Lorand Univ, Dept Pharmaceut Chem, H-1117 Budapest, Hungary
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2004年 / 378卷 / 06期
关键词：
microscopic protonation constant; NMR-pH titration; chemical shift; microspeciation;
D O I：
10.1007/s00216-003-2390-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The theory and practice of proton microspeciation based on NMR-pH titrations are surveyed. Principles of bi-, tri-, tetra-, and n-protic microequilibrium systems are discussed. Evaluation methods are exemplified by case studies on bi- and tetraprotic biomolecules. Selection criteria and proper-ties of 'reporter' NMR nuclei are described. Literature data on complete microspeciations of small ligands and site-specific basicity characterizations of peptides and proteins are critically reviewed.
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收藏
页码：1428 / 1448
页数：21
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