Paramagnetic fluorescence quenching in a model membrane: a consideration of lifetime and temperature

被引：8

作者：

Johnson, DA ^{[1
]}

Nguyen, B ^{[1
]}

Bohorquez, AF ^{[1
]}

Valenzuela, CF ^{[1
]}

机构：

[1] Univ Calif Riverside, Div Biomed Sci, Riverside, CA 92521 USA

来源：

BIOPHYSICAL CHEMISTRY | 1999年 / 79卷 / 01期

基金：

美国国家科学基金会;

关键词：

paramagnetic fluorescence quenching; fluorescent probe; spin label; liposomes; time-resolved fluorescence;

D O I：

10.1016/S0301-4622(99)00036-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To expand our understanding of paramagnetic quenching in membranes, the relationship between fluorophore excited-state lifetime (tau), temperature, and the collisional quenching was studied. Specifically, the ability of tempo to quench the steady-state and time-resolved emission from five lipophilic fluorophores (diphenylhexatriene, perylene, phenanthrene, pyrene, and triphenylene) partitioned into egg phosphatidylcholine (EggPC) liposomes was examined. Also, the temperature dependence of spin-labeled androstane to quench the emission (steady-state and time-resolved) from perylene in EggPC liposomes was determined. Unexpectedly, in EggPC liposomes, the apparent quenching efficiency decreased with increasing tau until the effect leveled off above similar to 20 ns. Moreover, in EggPC liposomes, dynamic quenching decreased with increasing temperature. The results suggest that in membranes, paramagnetic quenching is more complex than generally recognized. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：1 / 9

页数：9

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