Molecular Cloning and Characterization of Two Genes Encoding Dihydroflavonol-4-Reductase from Populus trichocarpa

被引：44

作者：

Huang, Yan ^{[1
,2
]}

Gou, Jiqing ^{[3
]}

Jia, Zhichun ^{[1
]}

Yang, Li ^{[1
]}

Sun, Yimin ^{[1
]}

Xiao, Xunyan ^{[1
]}

Song, Feng ^{[1
]}

Luo, Keming ^{[1
,2
]}

机构：

[1] Southwest Univ, Sch Life Sci, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Minist Educ,Inst Resources Bot, Chongqing, Peoples R China

[2] Southwest Univ, Chongqing Key Lab Transgen Plant & Safety Control, Chongqing, Peoples R China

[3] Samuel Roberts Noble Fdn Inc, Forage Improvement Div, Ardmore, OK USA

来源：

PLOS ONE | 2012年 / 7卷 / 02期

基金：

中国国家自然科学基金;

关键词：

FLAVONOID BIOSYNTHESIS; MEDICAGO-TRUNCATULA; CHEMICAL DEFENSE; TREMBLING ASPEN; EXPRESSION; 4-REDUCTASE; REDUCTASE; TANNIN; ARABIDOPSIS; METABOLISM;

D O I：

10.1371/journal.pone.0030364

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Dihydroflavonol 4-reductase (DFR, EC 1.1.1.219) is a rate-limited enzyme in the biosynthesis of anthocyanins and condensed tannins (proanthocyanidins) that catalyzes the reduction of dihydroflavonols to leucoanthocyanins. In this study, two full-length transcripts encoding for PtrDFR1 and PtrDFR2 were isolated from Populus trichocarpa. Sequence alignment of the two PtrDFRs with other known DFRs reveals the homology of these genes. The expression profile of PtrDFRs was investigated in various tissues of P. trichocarpa. To determine their functions, two PtrDFRs were overexpressed in tobacco (Nicotiana tabacum) via Agrobacterium-mediated transformation. The associated color change in the flowers was observed in all 35S:PtrDFR1 lines, but not in 35S: PtrDFR2 lines. Compared to the wild-type control, a significantly higher accumulation of anthocyanins was detected in transgenic plants harboring the PtrDFR1. Furthermore, overexpressing PtrDFR1 in Chinese white poplar (P. tomentosa Carr.) resulted in a higher accumulation of both anthocyanins and condensed tannins, whereas constitutively expressing PtrDFR2 only improved condensed tannin accumulation, indicating the potential regulation of condensed tannins by PtrDFR2 in the biosynthetic pathway in poplars.

引用

页数：8

共 51 条

[31] Two differentially regulated Arabidopsis genes define a new branch of the DFR superfamily [J].