Recombinant DNA production of spider silk proteins

被引：120
|
作者：
Tokareva, Olena [1 ]
Michalczechen-Lacerda, Valquiria A. [2 ]
Rech, Elibio L. [3 ]
Kaplan, David L. [1 ]
机构：
[1] Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA
[2] Univ Brasilia, Inst Biol, Dept Cell Biol, BR-70910900 Brasilia, DF, Brazil
[3] Embrapa Genet Resources & Biotechnol, Biotechnol Unit, BR-70770900 Brasilia, DF, Brazil
来源：
MICROBIAL BIOTECHNOLOGY | 2013年 / 6卷 / 06期
关键词：
DRAGLINE SILK; ESCHERICHIA-COLI; FIBERS; EXPRESSION; PURIFICATION; SPIN; FLAGELLIFORM; LARVAE; CELLS;
D O I：
10.1111/1751-7915.12081
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Spider dragline silk is considered to be the toughest biopolymer on Earth due to an extraordinary combination of strength and elasticity. Moreover, silks are biocompatible and biodegradable protein-based materials. Recent advances in genetic engineering make it possible to produce recombinant silks in heterologous hosts, opening up opportunities for large-scale production of recombinant silks for various biomedical and material science applications. We review the current strategies to produce recombinant spider silks.
引用
收藏
页码：651 / 663
页数：13
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