Assessment of the cytotoxicity of a mineral trioxide aggregate-based sealer with respect to macrophage activity

被引：19
作者：
Braga, Julia Mourao [1 ]
Oliveira, Ricardo Reis [1 ]
Martins, Renata de Castro [2 ]
Vieira, Leda Quercia [3 ,4 ]
Ribeiro Sobrinho, Antonio Paulino [1 ]
机构：
[1] Univ Fed Minas Gerais, Fac Odontol, Dept Odontol Restauradora, BR-30350260 Belo Horizonte, MG, Brazil
[2] Univ Fed Minas Gerais, Fac Odontol, Dept Odontol Social & Prevent, BR-30350260 Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, BR-30350260 Belo Horizonte, MG, Brazil
[4] Univ Fed Ouro Preto, Nucleo Pesquisa Ciencias Biol, Ouro Preto, MG, Brazil
来源：
DENTAL TRAUMATOLOGY | 2015年 / 31卷 / 05期
关键词：
macrophage; mineral trioxide aggregate; MTA Fillapex; Viability; Adherence; phagocytosis; END FILLING MATERIALS; IN-VITRO; MEDIATED APOPTOSIS; REACTIVE OXYGEN; TISSUE REACTION; ACTIVATION; ADHESION; IMMUNITY; BINDING; CELL;
D O I：
10.1111/edt.12190
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Aim: To assess the influence of co-culture with mineral trioxide aggregate (MTA) and MTA Fillapex (FLPX) on the viability, adherence, and phagocytosis activity of peritoneal macrophages from two mouse strains. Methodology: Cellular viability, adherence, and phagocytosis of Saccharomyces boulardii were assayed in the presence of capillaries containing MTA and MTA Fillapex. The data were analyzed using parametric (Student's t) and non-parametric (Mann-Whitney) tests. Results: FLPX was severely cytotoxic and decreased cell viability, adherence, and phagocytic activity of both macrophage subtypes. Cells that were treated with MTA Fillapex remained viable (>80%) for only 4 h after stimulation. Macrophages from C57BL/6 mice presented higher adherence and higher phagocytic activity compared with macrophages from BALB/c mice. Conclusion: Comparison of MTA and FLPX effects upon macrophages indicates that FLPX may impair macrophage activity and viability, while MTA seems to increase phagocytic activity.
引用
收藏
页码：390 / 395
页数：6
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