Effect of administered ethanol on protein kinase C in human platelets

被引：15

作者：

Deitrich, RA ^{[1
]}

Bludeau, P ^{[1
]}

Elk, ME ^{[1
]}

Baker, R ^{[1
]}

Menez, JF ^{[1
]}

Gill, K ^{[1
]}

机构：

[1] UNIV COLORADO,HLTH SCI CTR,ALCOHOL RES CTR,DENVER,CO 80262

来源：

ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH | 1996年 / 20卷 / 09期

关键词：

protein kinase C; ethanol; human platelets;

D O I：

10.1111/j.1530-0277.1996.tb01690.x

中图分类号：

R194 [卫生标准、卫生检查、医药管理];

学科分类号：

摘要：

There are numerous reports of the effect of ethanol on protein kinase C (PKC) in animals or with in vitro systems. However, the effect of ethanol on PKC in humans has not been extensively investigated despite the large number of studies involving PKC and human platelets. In this study, we administered ethanol to human volunteers and determined the level of PKC before and after a 0.4 g/kg dose of ethanol. We studied Native Americans and Caucasians of both sexes. There was an increase in PKC activity 60 min after ethanol administration. There were no ethnic, age, nor gender differences detected, nor was there any correlation between family history of alcoholism and the basal or stimulated platelet PKC levels. Neither was there any correlation of basal or stimulated PKC activity with the genotypes for ADH2, ADH3, ALDH2, CYP2E1, and CYP1A2.

引用

页码：1503 / 1506

页数：4

共 26 条

[1]

BAKER RC, 1990, J PHARMACOL EXP THER, V252, P1028

[2] EFFECTS OF ETHANOL ON HUMAN-PLATELETS STIMULATED WITH PLATELET-ACTIVATING FACTOR, A BIOLOGICALLY-ACTIVE ETHER PHOSPHOLIPID [J].

BAKER, RC ;

FISH, HT ;

FITZPATRICK, FA .

ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 1989, 13 (06) :824-828

[3]

BALDASSARE JJ, 1992, J BIOL CHEM, V267, P15585

[4] PROTEIN-KINASE-C INTERACTION WITH CALCIUM - A PHOSPHOLIPID-DEPENDENT PROCESS [J].

BAZZI, MD ;

NELSESTUEN, GL .

BIOCHEMISTRY, 1990, 29 (33) :7624-7630

[5] ETHANOL INHIBITS THROMBIN-INDUCED SECRETION BY HUMAN PLATELETS AT A SITE DISTINCT FROM PHOSPHOLIPASE-C OR PROTEIN KINASE-C [J].

BENISTANT, C ;

RUBIN, R .

BIOCHEMICAL JOURNAL, 1990, 269 (02) :489-497

[6] PLATELET PROTEIN-PHOSPHORYLATION AND PROTEIN KINASE-C ACTIVATION BY PHORBOL ESTERS WITH DIFFERENT BIOLOGICAL-ACTIVITY AND A NOVEL SYNERGISTIC RESPONSE WITH CA-2+ IONOPHORE [J].

BROOKS, SF ;

GORDGE, PC ;

TOKER, A ;

EVANS, AT ;

EVANS, FJ ;

AITKEN, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 188 (02) :431-437

[7]

CHANG JD, 1993, J BIOL CHEM, V268, P14208

[8] INVESTIGATIONS OF THE ROLE OF PROTEIN KINASE-C IN THE ACUTE SEDATIVE EFFECTS OF ETHANOL [J].

DEITRICH, RA ;

BLUDEAU, PA ;

BAKER, RC .

ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 1989, 13 (06) :737-745

[9] ETHANOL EXPOSURE RESULTS IN A TRANSIENT DECREASE IN HUMAN PLATELET CAMP LEVELS - EVIDENCE FOR A PROTEIN-KINASE-C MEDIATED PROCESS [J].

DEPETRILLO, PB ;

SWIFT, RM .

ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 1992, 16 (02) :290-294

[10] ETHANOL EXPOSURE INCREASES TOTAL PROTEIN-KINASE-C ACTIVITY IN HUMAN-LYMPHOCYTES [J].

DEPETRILLO, PB ;

LIOU, CS .

ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 1993, 17 (02) :351-354

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