Biotechnological Production of Odor-Active Methyl-Branched Aldehydes by a Novel α-Dioxygenase from Crocosphaera subtropica

被引：19

作者：

Hammer, Andreas K. ^{[1
]}

Albrecht, Florian ^{[1
]}

Hahne, Friederike ^{[1
]}

Jordan, Paulina ^{[2
]}

Fraatz, Marco A. ^{[1
,3
]}

Ley, Jakob ^{[4
]}

Geissler, Torsten ^{[4
]}

Schrader, Jens ^{[2
]}

Zorn, Holger ^{[1
,3
]}

Buchhaupt, Markus ^{[2
]}

机构：

[1] Justus Liebig Univ Giessen, Inst Food Chem & Food Biotechnol, Heinrich Buff Ring 17, D-35392 Giessen, Germany

[2] DECHEMA Res Inst, Ind Biotechnol, D-60486 Frankfurt, Germany

[3] Fraunhofer Inst Mol Biol & Appl Ecol, D-35394 Giessen, Germany

[4] Symrise AG, D-37603 Holzminden, Germany

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2020年 / 68卷 / 38期

关键词：

methyl-branched aldehydes; flavoring substances; alpha-dioxygenase; Crocosphaera subtropica (former Cyanothece sp. ATCC 51142); odor threshold; FATTY-ACIDS; HIGHER-PLANTS; OXIDATION; IDENTIFICATION; OXYGENASE; ENZYME; BIOSYNTHESIS; PEROXIDASE; ANALOGS; GREEN;

D O I：

10.1021/acs.jafc.0c02035

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

As a result of their pleasant odor qualities and low odor thresholds, iso- and anteiso-fatty aldehydes represent promising candidates for applications in flavoring preparations. A novel cyanobacterial alpha-dioxygenase from Crocosphaera subtropica was heterologously expressed in Escherichia coli and applied for the biotechnological production of C-12-C-15 branched-chain fatty aldehydes. The enzyme has a sequence identity of less than 40% to well-investigated alpha-dioxygenase from rice. Contrary to the latter, it efficiently transformed short-chained fatty acids. The kinetic parameters of alpha-dioxygenase toward unbranched and iso-branched-chain substrates were studied by means of an oxygen-depletion assay. The transformation products (C-12-C-15 iso- and anteiso-aldehydes) were extensively characterized, including their sensory properties. The aldehydes exhibited green-soapy, sweety odors with partial citrus-like, metallic, peppery, and savory-tallowy nuances. Moreover, the two C-14 isomers showed particularly low odor threshold values of 0.2 and 0.3 ng/L in air as determined by means of gas chromatography-olfactometry.

引用

页码：10432 / 10440

页数：9

共 51 条

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