Amphotericin B-copper(II) complex as a potential agent with higher antifungal activity against Candida albicans

被引：44

作者：

Chudzik, Barbara ^{[1
]}

Tracz, Izabela B. ^{[1
]}

Czernel, Grzegorz ^{[2
]}

Fiolka, Marta J. ^{[3
]}

Borsuk, Grzegorz ^{[4
]}

Gagos, Mariusz ^{[1
,2
]}

机构：

[1] Marie Curie Sklodowska Univ, Inst Biol & Biotechnol, Dept Cell Biol, PL-20033 Lublin, Poland

[2] Univ Life Sci Lublin, Dept Biophys, PL-20950 Lublin, Poland

[3] Marie Curie Sklodowska Univ, Inst Biol & Biochem, Dept Immunobiol, PL-20033 Lublin, Poland

[4] Univ Life Sci Lublin, Fac Biol & Anim Breeding, Dept Biol Basis Anim Prod, PL-20950 Lublin, Poland

来源：

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES | 2013年 / 49卷 / 05期

关键词：

Amphotericin B; Amphotericin B copper(II) complex; Candida albicans; Antifungal activity; COPPER II COMPLEXES; DIVALENT METAL-IONS; SACCHAROMYCES-CEREVISIAE; POLYENE ANTIBIOTICS; LIPID-MEMBRANES; CIRCULAR-DICHROISM; ESCHERICHIA-COLI; TRANSPORT; TOXICITY; CU(II);

D O I：

10.1016/j.ejps.2013.06.007

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Amphotericin B (AmB) is a polyene antibiotic produced by Streptomyces nodosus used for more than 50 years in the treatment of acute systemic fungal infections. It exhibits a broad spectrum of activity against fungal and protozoan pathogens with relatively rare resistance. The aim of this study was to prepare and evaluate the utility of the AmB-Cu2+ complex as a potential compound with a high fungicidal activity at lower concentrations, compared with conventional AmB. It was hypothesized that insertion of copper ions into fungal cell membranes, together with the AmB-Cu2+ complex bypassing the natural homeostatic mechanisms of this element, may contribute to the increased fungicidal activity of AmB. The analysis of results indicates the increased antifungal activity of the AmB-Cu2+ complex against Candida albicans in comparison with the pure AmB and Fungizone. Additionally, it was stated that the increased antifungal activity of the AmB-Cu2+ complex is not the sum of the toxic effects of AmB and Cu2+ ions, but is a result of the unique structure of this compound. (c) 2013 Elsevier B.V. All rights reserved.

引用

页码：850 / 857

页数：8

共 55 条

[1] Genome-wide expression profiling of the response to polyene, pyrimidine, azole, and echinocandin antifungal agents in Saccharomyces cerevisiae [J].

Agarwal, AK ;

Rogers, PD ;

Baerson, SR ;

Jacob, MR ;

Barker, KS ;

Cleary, JD ;

Walker, LA ;

Nagle, DG ;

Clark, AM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (37) :34998-35015

[2]

[Anonymous], 2002, CLSI document M27-A2, V2nd

[3] RESPIRATORY BURST AND CANDIDACIDAL ACTIVITY OF PERITONEAL-MACROPHAGES ARE IMPAIRED IN COPPER-DEFICIENT RATS [J].

BABU, U ;

FAILLA, ML .

JOURNAL OF NUTRITION, 1990, 120 (12) :1692-1699

[4] Comparative molecular dynamics simulations of amphotericin B-cholesterol/ergosterol membrane channels [J].

Baginski, M ;

Resat, H ;

Borowski, E .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2002, 1567 (1-2) :63-78

[5]

Baginski M, 2006, ADV PLANAR LIPID BIL, P269

[6] EFFECTS OF THE AGGREGATION STATE OF AMPHOTERICIN-B ON ITS TOXICITY TO MICE [J].

BARWICZ, J ;

CHRISTIAN, S ;

GRUDA, I .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1992, 36 (10) :2310-2315

[7] COMPLEXES OF POLYENE ANTIBIOTICS WITH DIVALENT METAL-IONS - COMPLEXES OF CU(II) WITH NYSTATIN STUDIED BY VISIBLE CIRCULAR-DICHROISM [J].

BEEZER, AE ;

OBRIEN, P .

JOURNAL OF INORGANIC & NUCLEAR CHEMISTRY, 1981, 43 (07) :1669-1670

[8] THE SYNTHESIS AND CHARACTERIZATION OF POLYENE COMPLEXES WITH DIVALENT METAL-IONS - MG(II), CA(II), NI(II), CU(II) AND ZN(II) [J].

BEEZER, AE ;

OBRIEN, P ;

SHAM, WL .

INORGANICA CHIMICA ACTA-BIOINORGANIC CHEMISTRY, 1985, 108 (02) :117-122

[9] STABILITY-CONSTANTS FOR, AND STRUCTURAL INVESTIGATION OF, DIVALENT METAL-ION COMPLEXES WITH POLYENE ANTIBIOTICS [J].

BEEZER, AE ;

OBRIEN, P ;

SHAM, WL .

INORGANICA CHIMICA ACTA-BIOINORGANIC CHEMISTRY, 1985, 108 (02) :123-127

[10]

Belkherroubi-Sari L, 2008, AFR J MICROBIOL RES, V2, P242

← 1 2 3 4 5 6 →