Comparison of Absorption Characteristics of Iron Glycine Chelate and Ferrous Sulfate in Caco-2 Cells

被引：0
作者：
Ma, Wen-Qiang [1 ,2 ,3 ]
Wu, Jing [1 ,2 ]
Zhuo, Zhao [1 ,2 ]
Sun, Hong [1 ,2 ]
Yue, Min [1 ,2 ]
Feng, Jie [1 ,2 ]
机构：
[1] Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Zhejiang, Peoples R China
[2] Zhejiang Univ, Coll Anim Sci, Minist Educ, Key Lab Mol Anim Nutr, Hangzhou 310029, Zhejiang, Peoples R China
[3] Nanjing Agr Univ, Coll Vet Med, Key Lab Anim Physiol & Biochem, Nanjing 210095, Jiangsu, Peoples R China
来源：
INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY | 2013年 / 15卷 / 02期
关键词：
Fe-Gly; FeSO4; Caco-2; Cell; Concentration; Time; Temperature; DEFICIENCY ANEMIA; WEANLING PIGS; BIOAVAILABILITY; GROWTH; DRUGS; MODEL; AVAILABILITY; TRANSPORT; TOXICITY; CULTURE;
D O I：
暂无
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The study was conducted to compare the absorption characteristics of iron glycine chelate (Fe-Gly) and ferrous sulfate (FeSO4) in Caco-2 cells. Effects of several factors, including concentration, time and temperature on iron (Fe) transport were investigated. In Caco-2 cell model, Fe transport amount in Fe-Gly treatment was higher than that in Fe SO4 from the apical side (AP) to the basolateral (BL) side. However, there was no significant difference between the FeSO4 and Fe-Gly treatments in the direction of BL to AP. Similar results were also found on Fe transport rate of Fe-Gly and FeSO4 across Caco-2 monolayers. The apparent permeability coefficient of Fe-Gly was significantly higher than that of Fe SO4 in the direction of AP to BL. The ratio of the apparent permeability coefficient between AP to BL and BL to AP was greater than 1.0 for both forms of Fe resources. The Fe transport amount in Fe-Gly treatment was higher than that in FeSO4 treatment, no matter the incubation temperature was 37 degrees C or 4 degrees C. However, it significantly decreased, when the incubation temperature dropped from 37 degrees C to 4 degrees C. The results indicate that Fe of Fe-Gly can be easily absorbed than FeSO4 in Caco2 cells. (c) 2013 Friends Science Publishers
引用
收藏
页码：372 / 376
页数：5
相关论文
共 30 条
[1]   Advantages and limitations of iron amino acid chelates as iron fortificants [J].
Allen, LH .
NUTRITION REVIEWS, 2002, 60 (07) :S18-S21
[2]   EPITHELIAL TRANSPORT OF DRUGS IN CELL-CULTURE .1. A MODEL FOR STUDYING THE PASSIVE DIFFUSION OF DRUGS OVER INTESTINAL ABSORPTIVE (CACO-2) CELLS [J].
ARTURSSON, P .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1990, 79 (06) :476-482
[3]  
Ashmead SD, 2001, ARCH LATINOAM NUTR, V51, P7
[4]   Effects of iron glycine chelate on growth, haematological and immunological characteristics in weanling pigs [J].
Feng, J. ;
Ma, W. Q. ;
Xu, Z. R. ;
Wang, Y. Z. ;
Liu, J. X. .
ANIMAL FEED SCIENCE AND TECHNOLOGY, 2007, 134 (3-4) :261-272
[5]   The effect of iron glycine chelate on tissue mineral levels, fecal mineral concentration, and liver antioxidant enzyme activity in weanling pigs [J].
Feng, J. ;
Ma, W. Q. ;
Xu, Z. R. ;
He, J. X. ;
Wang, Y. Z. ;
Liu, J. X. .
ANIMAL FEED SCIENCE AND TECHNOLOGY, 2009, 150 (1-2) :106-113
[6]   Bioavailability of iron glycine as a fortificant in infant foods [J].
Fox, TE ;
Eagles, J ;
Fairweather-Tait, SJ .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 1998, 67 (04) :664-668
[7]   Applications of the Caco-2 model in the design and development of orally active drugs: Elucidation of biochemical and physical barriers posed by the intestinal epithelium [J].
Gan, LSL ;
Thakker, DR .
ADVANCED DRUG DELIVERY REVIEWS, 1997, 23 (1-3) :77-98
[8]  
Giorgini E, 2001, ARCH LATINOAM NUTR, V51, P48
[9]   Comparison of iron bioavallability from 15 rice genotypes: Studies using an in vitro digestion/caco-2 cell culture model [J].
Glahn, RP ;
Chen, SQ ;
Welch, RM ;
Gregorio, GB .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (12) :3586-3591
[10]   Iron absorption from the whole diet in men: How effective is the regulation of iron absorption [J].
Hallberg, L ;
Hulten, L ;
Gramatkovski, E .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 1997, 66 (02) :347-356
123